Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3199935..3200566 Replicon chromosome
Accession NZ_CP014583
Organism Escherichia coli strain CFSAN004176

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag BCV59_RS17340 Protein ID WP_001259385.1
Coordinates 3199935..3200210 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BCV59_RS17345 Protein ID WP_000593555.1
Coordinates 3200207..3200566 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCV59_RS17325 3194939..3196006 + 1068 WP_000361467.1 HlyD family secretion protein -
BCV59_RS17330 3196003..3198738 + 2736 WP_000149164.1 ribosome-associated ATPase/putative transporter RbbA -
BCV59_RS17335 3198738..3199862 + 1125 WP_001216257.1 ABC transporter permease -
BCV59_RS17340 3199935..3200210 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
BCV59_RS17345 3200207..3200566 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BCV59_RS17350 3200686..3201087 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
BCV59_RS17355 3201093..3201899 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
BCV59_RS17360 3201896..3202660 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
BCV59_RS17365 3202660..3203493 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
BCV59_RS17370 3203490..3204434 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3192951..3201087 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T61533 WP_001259385.1 NZ_CP014583:3199935-3200210 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T61533 NZ_CP014583:3199935-3200210 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT61533 WP_000593555.1 NZ_CP014583:3200207-3200566 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT61533 NZ_CP014583:3200207-3200566 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References