Detailed information of TA system TA061528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 806278..806756
• Replicon: chromosome
• Accession: NZ_CP014583
• Organism: Escherichia coli strain CFSAN004176

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A7D8WGY6
• Locus tag: BCV59_RS05490
• Protein ID: WP_000936798.1
• Coordinates: 806469..806756 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: D8A5B4
• Locus tag: BCV59_RS05485
• Protein ID: WP_000100899.1
• Coordinates: 806278..806469 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV59_RS05435	801340..801696	-	357	WP_069357105.1	hypothetical protein	-
BCV59_RS05440	801698..802159	-	462	WP_069357106.1	ead/Ea22-like family protein	-
BCV59_RS05445	802146..802379	-	234	WP_000699805.1	hypothetical protein	-
BCV59_RS05450	802376..802657	-	282	WP_077886885.1	DNA-binding protein	-
BCV59_RS05455	802690..803457	-	768	WP_069357108.1	DUF1627 domain-containing protein	-
BCV59_RS30135	803492..804034	-	543	WP_157917783.1	replication protein	-
BCV59_RS30940	803946..804986	-	1041	WP_069357109.1	phage replisome organizer	-
BCV59_RS05470	805058..805483	-	426	WP_069357110.1	Rha family transcriptional regulator	-
BCV59_RS05475	805467..805739	-	273	WP_069357111.1	helix-turn-helix domain-containing protein	-
BCV59_RS05480	805849..806250	+	402	WP_069357112.1	helix-turn-helix domain-containing protein	-
BCV59_RS05485	806278..806469	+	192	WP_000100899.1	hypothetical protein	Antitoxin
BCV59_RS05490	806469..806756	+	288	WP_000936798.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BCV59_RS05495	807031..807183	+	153	WP_021523420.1	DUF1391 family protein	-
BCV59_RS05500	807195..807554	+	360	WP_172907136.1	hypothetical protein	-
BCV59_RS05510	808283..808471	+	189	WP_069357115.1	cell division inhibitor	-
BCV59_RS05515	808468..808656	+	189	WP_000199478.1	DUF1482 family protein	-
BCV59_RS05520	808749..811187	+	2439	WP_069357116.1	exonuclease	-
BCV59_RS05525	811249..811455	+	207	WP_069357117.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	778634..819754	41120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10899.83 Da
• Isoelectric Point: 10.1360

>T61528 WP_000936798.1 NZ_CP014583:806469-806756 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENIPAARRLKIRIETSVLALSEHPYLYPPSDRVSGLREIVVHPNYIVLYRVAASSIE
IANIVHARRQFPFPI

Nucleotide

• Length: 288 bp

>T61528 NZ_CP014583:806469-806756 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCATCTGCTCGCGATGATTTGCGTCAGATTGTAGCCTATATTGCTAAGGAAAATATTCC
TGCAGCACGCAGACTAAAAATACGTATTGAAACGTCTGTTTTAGCCCTCTCCGAGCATCCATATCTATATCCGCCAAGTG
ATCGAGTATCCGGTTTGCGGGAAATTGTGGTTCACCCTAATTATATCGTTTTGTACCGAGTAGCAGCTTCAAGCATTGAA
ATTGCAAATATTGTGCATGCCCGCCGACAATTTCCCTTCCCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7035.80 Da
• Isoelectric Point: 4.0270

>AT61528 WP_000100899.1 NZ_CP014583:806278-806469 [Escherichia coli]
MSTALSPIVSEFETTEQENSYNEWLRAKVASSLADPRPSIPHDEVMAEMENLIAQIAVTNRSE

Nucleotide

• Length: 192 bp

>AT61528 NZ_CP014583:806278-806469 [Escherichia coli]
ATGAGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCGC
TAAAGTGGCGTCAAGCCTTGCAGACCCTCGTCCCTCAATTCCACATGACGAGGTAATGGCTGAAATGGAGAATCTTATTG
CTCAAATTGCTGTAACTAACAGGAGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7D8WGY6

Antitoxin

• AlphaFold DB: A0A161RC07