Detailed information of TA system TA061517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParST/RES-TIGR02293
• Location: 98718..99643
• Replicon: plasmid pOLGA2
• Accession: NZ_CP014581
• Organism: Paraburkholderia phytofirmans OLGA172

Toxin (Protein)

• Gene name: ParT
• Uniprot ID: A0A167WR65
• Locus tag: AYM40_RS37530
• Protein ID: WP_063501221.1
• Coordinates: 98718..99197 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ParS
• Uniprot ID: -
• Locus tag: AYM40_RS37535
• Protein ID: WP_082855584.1
• Coordinates: 99194..99643 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYM40_RS37500	94531..95229	+	699	WP_063501258.1	translesion DNA synthesis-associated protein ImuA	-
AYM40_RS37505	95487..95915	-	429	WP_063501259.1	hypothetical protein	-
AYM40_RS37510	95960..97054	-	1095	WP_063501217.1	hypothetical protein	-
AYM40_RS37515	97075..97767	-	693	WP_063501218.1	SOS response-associated peptidase	-
AYM40_RS37520	97794..98093	-	300	WP_063501219.1	hypothetical protein	-
AYM40_RS37525	98297..98644	-	348	WP_063501220.1	hypothetical protein	-
AYM40_RS37530	98718..99197	-	480	WP_063501221.1	RES family NAD+ phosphorylase	Toxin
AYM40_RS37535	99194..99643	-	450	WP_082855584.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
AYM40_RS37540	99869..100171	+	303	WP_063501223.1	hypothetical protein	-
AYM40_RS41150	100653..100898	-	246	Protein_118	hypothetical protein	-
AYM40_RS37550	101103..101903	-	801	WP_063501225.1	IS21-like element helper ATPase IstB	-
AYM40_RS37555	101896..103419	-	1524	WP_063501260.1	IS21 family transposase	-
AYM40_RS37560	103536..104081	-	546	Protein_121	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..137323	137323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (5-144)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (101-146)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17231.74 Da
• Isoelectric Point: 5.7367

>T61517 WP_063501221.1 NZ_CP014581:c99197-98718 [Paraburkholderia phytofirmans OLGA172]
MTVAIWRIATDTPDYTADDLSGEGAKRTGGRWNRAGMPVLYCASSIALACLETVVHWNAGGLPLNRYLVRIDVPDEVWNA
ASIVTADAGPVGWDAIPEGKVSLDLGDGWISAKKSALLLVPSVIVPEEFNALVNPLHPDASTFTATKLRKWLYDSRIKK

Nucleotide

• Length: 480 bp

>T61517 NZ_CP014581:c99197-98718 [Paraburkholderia phytofirmans OLGA172]
ATGACGGTGGCGATCTGGCGGATTGCCACTGACACTCCCGATTACACCGCCGATGACCTGTCTGGCGAGGGCGCCAAGCG
AACCGGTGGCCGATGGAACAGGGCAGGTATGCCGGTGCTTTACTGCGCGTCGAGCATTGCGCTGGCTTGCCTTGAAACCG
TCGTGCATTGGAATGCCGGGGGTCTGCCTTTGAACCGGTATCTAGTTCGGATAGATGTGCCGGATGAAGTTTGGAACGCC
GCGTCGATCGTGACGGCCGACGCGGGTCCGGTCGGTTGGGATGCCATACCTGAAGGCAAGGTAAGTCTCGACCTCGGCGA
CGGCTGGATCAGCGCTAAGAAGTCAGCGCTTTTGCTGGTGCCATCAGTGATCGTTCCTGAAGAATTCAACGCGCTTGTAA
ATCCGTTGCATCCGGACGCCTCCACTTTCACCGCGACGAAGCTGCGCAAATGGCTCTATGACAGCCGAATCAAGAAGTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16169.51 Da
• Isoelectric Point: 5.2456

>AT61517 WP_082855584.1 NZ_CP014581:c99643-99194 [Paraburkholderia phytofirmans OLGA172]
MGRTPRTIGDFVQIYEAGPMERIELIKSGISADGLVDLATSMHTTKDQLFKRLGLPRATVDRKARNKQNLSQEQSERVIG
LMKLVGQVKTMVEQSGEPEGFDAAQWVGQWLEQANGALGGIPPAELMDTVQGQEVIAGLLAKMQSGAYA

Nucleotide

• Length: 450 bp

>AT61517 NZ_CP014581:c99643-99194 [Paraburkholderia phytofirmans OLGA172]
ATGGGCCGCACGCCCCGAACGATCGGCGACTTCGTGCAGATTTACGAAGCGGGTCCAATGGAACGGATCGAGCTAATCAA
AAGTGGAATCAGCGCCGATGGGCTTGTTGATCTGGCGACGTCAATGCATACGACAAAGGACCAGCTCTTCAAGCGGCTTG
GGCTTCCGCGTGCGACGGTCGATCGCAAGGCACGTAACAAGCAAAACCTTTCGCAGGAGCAGTCAGAGCGCGTCATTGGC
CTGATGAAGCTCGTCGGCCAGGTGAAGACGATGGTCGAGCAATCGGGCGAGCCGGAAGGCTTTGACGCCGCTCAATGGGT
TGGACAGTGGCTTGAGCAAGCGAACGGAGCGCTCGGGGGCATCCCCCCCGCCGAGCTGATGGACACGGTTCAAGGACAGG
AAGTGATCGCAGGTCTTCTCGCGAAGATGCAGAGCGGGGCATACGCATGA

Structures

Toxin

• AlphaFold DB: A0A167WR65