Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3821826..3822460 Replicon chromosome
Accession NZ_CP014578
Organism Paraburkholderia phytofirmans OLGA172

Toxin (Protein)


Gene name vapC Uniprot ID A0A160FMQ4
Locus tag AYM40_RS16755 Protein ID WP_063497186.1
Coordinates 3821826..3822227 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name VapB1 Uniprot ID A0A160FMT0
Locus tag AYM40_RS16760 Protein ID WP_063497187.1
Coordinates 3822227..3822460 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYM40_RS16735 3817674..3818144 - 471 WP_063498078.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
AYM40_RS16740 3818295..3819536 - 1242 WP_063497183.1 tRNA epoxyqueuosine(34) reductase QueG -
AYM40_RS16745 3819554..3820162 + 609 WP_063497184.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
AYM40_RS16750 3820144..3821730 + 1587 WP_063497185.1 N-acetylmuramoyl-L-alanine amidase -
AYM40_RS16755 3821826..3822227 - 402 WP_063497186.1 type II toxin-antitoxin system VapC family toxin Toxin
AYM40_RS16760 3822227..3822460 - 234 WP_063497187.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYM40_RS16765 3822630..3823505 + 876 WP_063497188.1 pirin family protein -
AYM40_RS16770 3823662..3824510 + 849 WP_063497189.1 thioredoxin -
AYM40_RS16775 3824692..3825549 - 858 WP_063497190.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -
AYM40_RS16780 3825794..3826435 + 642 WP_063497191.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-121)

Antitoxin

SpoVT-AbrB domain

(6-46)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14823.96 Da        Isoelectric Point: 4.9309

>T61506 WP_063497186.1 NZ_CP014578:c3822227-3821826 [Paraburkholderia phytofirmans OLGA172]
MPRYMLDTNICIYLMKNQPEQVAKRFAECFVGDVVMSAITFAELEYGVAVSANRARERRNLAALAEDILVMPFDSAAASA
YGPIREATREHKKDQFDELIAAHAVALGVVLVTNNERDFAAYPGLRVENWLNG

Download         Length: 402 bp

>T61506 NZ_CP014578:c3822227-3821826 [Paraburkholderia phytofirmans OLGA172]
ATGCCGCGCTACATGCTCGACACGAATATCTGCATCTACCTGATGAAGAACCAGCCGGAACAGGTTGCGAAGCGCTTTGC
GGAATGTTTCGTCGGCGATGTGGTGATGTCTGCCATTACTTTTGCAGAACTCGAATACGGCGTGGCGGTATCGGCGAACC
GTGCGCGCGAGCGCCGCAATCTGGCGGCACTCGCCGAAGATATTCTGGTCATGCCGTTCGACAGCGCTGCAGCAAGCGCG
TATGGTCCGATTCGCGAGGCGACGCGTGAGCATAAGAAGGATCAGTTCGACGAGCTAATCGCGGCACATGCCGTGGCGCT
CGGCGTGGTGCTGGTGACCAACAACGAACGTGACTTCGCCGCGTATCCGGGGCTGCGCGTGGAAAACTGGCTGAACGGCT
AG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8853.05 Da        Isoelectric Point: 5.8836

>AT61506 WP_063497187.1 NZ_CP014578:c3822460-3822227 [Paraburkholderia phytofirmans OLGA172]
MLTKVFRNGNSQAVRIPAELAFDRADVEVEIERVGEELRIRPTRRPLTGVLKKFAKFGPDFMSEGRGDHEQPEREDL

Download         Length: 234 bp

>AT61506 NZ_CP014578:c3822460-3822227 [Paraburkholderia phytofirmans OLGA172]
ATGCTCACCAAAGTCTTTCGCAACGGCAATTCGCAAGCGGTGCGTATTCCAGCCGAATTGGCATTCGACCGCGCCGACGT
CGAGGTTGAAATCGAACGAGTTGGCGAAGAATTGCGGATCCGTCCAACCCGGCGTCCGCTGACCGGCGTGCTCAAGAAAT
TCGCCAAATTCGGGCCAGACTTCATGTCCGAAGGACGTGGAGATCACGAGCAGCCCGAGCGCGAGGATTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A160FMQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A160FMT0

References