Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3694109..3694783 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag AZH48_RS17375 Protein ID WP_003417282.1
Coordinates 3694109..3694537 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag AZH48_RS17380 Protein ID WP_003417286.1
Coordinates 3694541..3694783 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS17350 3689931..3690332 - 402 WP_003417264.1 cytidine deaminase -
AZH48_RS17355 3690473..3690907 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
AZH48_RS17360 3690904..3691338 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
AZH48_RS17365 3691467..3693239 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
AZH48_RS17370 3693239..3694030 + 792 WP_010950870.1 succinate dehydrogenase iron-sulfur subunit -
AZH48_RS17375 3694109..3694537 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
AZH48_RS17380 3694541..3694783 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
AZH48_RS17385 3694905..3695519 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
AZH48_RS17390 3695516..3696181 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
AZH48_RS17395 3696182..3696715 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
AZH48_RS17400 3696712..3697086 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
AZH48_RS17405 3697183..3698247 - 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T61495 WP_003417282.1 NZ_CP014566:c3694537-3694109 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T61495 NZ_CP014566:c3694537-3694109 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT61495 WP_003417286.1 NZ_CP014566:c3694783-3694541 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT61495 NZ_CP014566:c3694783-3694541 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References