Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3129897..3130584 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FBY8
Locus tag AZH48_RS14830 Protein ID WP_011799298.1
Coordinates 3130141..3130584 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag AZH48_RS14825 Protein ID WP_003414620.1
Coordinates 3129897..3130154 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS14805 3125217..3126071 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
AZH48_RS14810 3126127..3127290 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
AZH48_RS14815 3127307..3128521 - 1215 WP_011799297.1 zinc metalloprotease Rip -
AZH48_RS14820 3128529..3129770 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
AZH48_RS14825 3129897..3130154 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
AZH48_RS14830 3130141..3130584 + 444 WP_011799298.1 type II toxin-antitoxin system VapC family toxin Toxin
AZH48_RS14835 3130664..3131326 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
AZH48_RS14840 3131422..3131613 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
AZH48_RS14845 3131606..3133693 + 2088 WP_003414635.1 cytochrome c biogenesis protein DipZ -
AZH48_RS14850 3133789..3134370 + 582 WP_003414644.1 fasciclin domain-containing protein -
AZH48_RS14855 3134422..3134736 + 315 WP_011799299.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16625.74 Da        Isoelectric Point: 6.4890

>T61493 WP_011799298.1 NZ_CP014566:3130141-3130584 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDTLLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T61493 NZ_CP014566:3130141-3130584 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACACCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT61493 WP_003414620.1 NZ_CP014566:3129897-3130154 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT61493 NZ_CP014566:3129897-3130154 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBY8

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References