Detailed information of TA system TA061489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3056388..3056958
• Replicon: chromosome
• Accession: NZ_CP014566
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: AZH48_RS14445
• Protein ID: WP_003414166.1
• Coordinates: 3056388..3056744 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: AZH48_RS14450
• Protein ID: WP_003901465.1
• Coordinates: 3056728..3056958 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZH48_RS14425	3051840..3053528	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
AZH48_RS14430	3053532..3053858	-	327	WP_003414157.1	hypothetical protein	-
AZH48_RS14435	3053989..3054618	+	630	WP_003414158.1	DUF3558 family protein	-
AZH48_RS14440	3054637..3056286	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
AZH48_RS14445	3056388..3056744	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
AZH48_RS14450	3056728..3056958	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
AZH48_RS14455	3057001..3058044	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
AZH48_RS21920	3058235..3058510	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
AZH48_RS22310	3058686..3058940	-	255	WP_003917684.1	hypothetical protein	-
AZH48_RS14470	3059088..3059492	+	405	WP_003414181.1	hypothetical protein	-
AZH48_RS14475	3059489..3059680	+	192	WP_003414184.1	hypothetical protein	-
AZH48_RS14480	3059879..3061033	+	1155	WP_011799292.1	DDE-type integrase/transposase/recombinase	-
AZH48_RS14485	3061267..3061524	+	258	WP_003899489.1	hypothetical protein	-
AZH48_RS14490	3061629..3061940	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T61489 WP_003414166.1 NZ_CP014566:c3056744-3056388 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T61489 NZ_CP014566:c3056744-3056388 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT61489 WP_003901465.1 NZ_CP014566:c3056958-3056728 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT61489 NZ_CP014566:c3056958-3056728 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3