Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2825831..2826462 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag AZH48_RS13215 Protein ID WP_003413174.1
Coordinates 2826085..2826462 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag AZH48_RS13210 Protein ID WP_003413167.1
Coordinates 2825831..2826088 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS13175 2820996..2821403 + 408 WP_003413029.1 hypothetical protein -
AZH48_RS13180 2821699..2822910 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
AZH48_RS13185 2823037..2823696 + 660 WP_003900846.1 LppA family lipoprotein -
AZH48_RS13190 2823693..2824352 + 660 WP_003900847.1 hypothetical protein -
AZH48_RS13195 2824349..2825011 + 663 WP_003900848.1 hypothetical protein -
AZH48_RS13200 2825008..2825286 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
AZH48_RS13205 2825379..2825792 + 414 WP_003413164.1 PIN domain nuclease -
AZH48_RS13210 2825831..2826088 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
AZH48_RS13215 2826085..2826462 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
AZH48_RS13220 2826478..2826852 - 375 WP_003413177.1 hypothetical protein -
AZH48_RS13225 2826952..2827347 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
AZH48_RS13230 2827344..2827589 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
AZH48_RS13235 2828000..2828419 - 420 WP_003413190.1 A24 family peptidase -
AZH48_RS13240 2828431..2829258 - 828 WP_010950736.1 shikimate dehydrogenase -
AZH48_RS13245 2829236..2830489 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
AZH48_RS13250 2830482..2830994 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T61484 WP_003413174.1 NZ_CP014566:2826085-2826462 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T61484 NZ_CP014566:2826085-2826462 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT61484 WP_003413167.1 NZ_CP014566:2825831-2826088 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT61484 NZ_CP014566:2825831-2826088 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References