Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2764652..2765304 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag AZH48_RS12915 Protein ID WP_003412752.1
Coordinates 2764879..2765304 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag AZH48_RS12910 Protein ID WP_003412749.1
Coordinates 2764652..2764873 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS12895 2762892..2763098 - 207 WP_003899347.1 hypothetical protein -
AZH48_RS12900 2763234..2763857 + 624 WP_003900858.1 TIGR00725 family protein -
AZH48_RS12905 2763847..2764599 + 753 WP_003901416.1 hypothetical protein -
AZH48_RS12910 2764652..2764873 + 222 WP_003412749.1 antitoxin Antitoxin
AZH48_RS12915 2764879..2765304 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
AZH48_RS12920 2765327..2766508 - 1182 WP_010950729.1 2-oxo acid dehydrogenase subunit E2 -
AZH48_RS12925 2766505..2767551 - 1047 WP_010950730.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
AZH48_RS12930 2767562..2768665 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
AZH48_RS12935 2768924..2769745 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
AZH48_RS12940 2769742..2770299 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T61481 WP_003412752.1 NZ_CP014566:2764879-2765304 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T61481 NZ_CP014566:2764879-2765304 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT61481 WP_003412749.1 NZ_CP014566:2764652-2764873 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT61481 NZ_CP014566:2764652-2764873 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References