Detailed information of TA system TA061473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2196844..2197532
• Replicon: chromosome
• Accession: NZ_CP014566
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: AZH48_RS10210
• Protein ID: WP_003409958.1
• Coordinates: 2196844..2197263 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: AZH48_RS10215
• Protein ID: WP_003409968.1
• Coordinates: 2197272..2197532 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZH48_RS10190	2192339..2193187	+	849	WP_003409942.1	class I SAM-dependent methyltransferase	-
AZH48_RS10195	2193150..2194595	-	1446	WP_003409946.1	APC family permease	-
AZH48_RS10200	2194774..2195460	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
AZH48_RS10205	2195651..2196619	-	969	WP_003409956.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
AZH48_RS10210	2196844..2197263	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
AZH48_RS10215	2197272..2197532	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AZH48_RS10220	2197675..2199351	+	1677	WP_010950639.1	PecA family PE domain-processing aspartic protease	-
AZH48_RS10225	2199339..2199992	-	654	WP_003409976.1	cutinase Cfp21	-
AZH48_RS10230	2200107..2200337	+	231	WP_003409980.1	DUF167 domain-containing protein	-
AZH48_RS10235	2200422..2201333	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
AZH48_RS10240	2201442..2202041	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T61473 WP_003409958.1 NZ_CP014566:c2197263-2196844 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T61473 NZ_CP014566:c2197263-2196844 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT61473 WP_003409968.1 NZ_CP014566:c2197532-2197272 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT61473 NZ_CP014566:c2197532-2197272 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483