Detailed information of TA system TA061470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2178793..2179496
• Replicon: chromosome
• Accession: NZ_CP014566
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: AZH48_RS10060
• Protein ID: WP_003409778.1
• Coordinates: 2178793..2179122 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: AZH48_RS20970
• Protein ID: WP_003409780.1
• Coordinates: 2179119..2179496 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZH48_RS10040	2175176..2176246	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
AZH48_RS10045	2176243..2176758	+	516	WP_003409718.1	flavin reductase family protein	-
AZH48_RS10050	2176755..2177816	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
AZH48_RS10055	2177813..2178583	+	771	WP_003409775.1	SDR family oxidoreductase	-
AZH48_RS10060	2178793..2179122	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AZH48_RS20970	2179119..2179496	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
AZH48_RS10070	2179493..2180083	-	591	WP_003910477.1	SEC-C domain-containing protein	-
AZH48_RS10075	2180138..2181502	+	1365	WP_011799233.1	HNH endonuclease	-
AZH48_RS10080	2181657..2182109	-	453	WP_010950636.1	lipoprotein	-
AZH48_RS10085	2182173..2182574	+	402	WP_003409869.1	hypothetical protein	-
AZH48_RS10090	2182567..2182749	-	183	WP_003409870.1	hypothetical protein	-
AZH48_RS10095	2182863..2183213	-	351	WP_003409871.1	hypothetical protein	-
AZH48_RS10100	2183224..2184126	-	903	WP_003409874.1	hypothetical protein	-
AZH48_RS10105	2184147..2184338	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T61470 WP_003409778.1 NZ_CP014566:c2179122-2178793 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T61470 NZ_CP014566:c2179122-2178793 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT61470 WP_003409780.1 NZ_CP014566:c2179496-2179119 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT61470 NZ_CP014566:c2179496-2179119 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8