Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1925146..1925759 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag AZH48_RS08935 Protein ID WP_003408465.1
Coordinates 1925146..1925535 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag AZH48_RS08940 Protein ID WP_003408469.1
Coordinates 1925532..1925759 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS08905 1920776..1921690 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
AZH48_RS08910 1921693..1922523 + 831 WP_003408448.1 cyclase family protein -
AZH48_RS08915 1922523..1922873 + 351 WP_003898986.1 cupin domain-containing protein -
AZH48_RS08920 1922926..1923743 + 818 Protein_1745 3-keto-5-aminohexanoate cleavage protein -
AZH48_RS08925 1923757..1924536 + 780 WP_003408460.1 IclR family transcriptional regulator -
AZH48_RS08935 1925146..1925535 - 390 WP_003408465.1 PIN domain-containing protein Toxin
AZH48_RS08940 1925532..1925759 - 228 WP_003408469.1 antitoxin Antitoxin
AZH48_RS08945 1925977..1927461 + 1485 WP_010950580.1 biotin carboxylase -
AZH48_RS08950 1927458..1928705 + 1248 WP_003408476.1 serine hydrolase -
AZH48_RS08955 1928748..1929167 - 420 WP_003408483.1 hypothetical protein -
AZH48_RS08960 1929157..1929867 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T61468 WP_003408465.1 NZ_CP014566:c1925535-1925146 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T61468 NZ_CP014566:c1925535-1925146 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT61468 WP_003408469.1 NZ_CP014566:c1925759-1925532 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT61468 NZ_CP014566:c1925759-1925532 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References