Detailed information of TA system TA061462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242168..1242736
• Replicon: chromosome
• Accession: NZ_CP014566
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: AZH48_RS05925
• Protein ID: WP_003405865.1
• Coordinates: 1242362..1242736 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: AZH48_RS05920
• Protein ID: WP_003405863.1
• Coordinates: 1242168..1242365 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZH48_RS05900	1238209..1238847	-	639	WP_011799158.1	lipid droplet-associated protein	-
AZH48_RS05905	1238937..1239944	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
AZH48_RS05910	1239961..1240944	-	984	WP_003898731.1	hypothetical protein	-
AZH48_RS05915	1241007..1242080	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
AZH48_RS05920	1242168..1242365	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AZH48_RS05925	1242362..1242736	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
AZH48_RS05930	1242939..1243637	+	699	WP_010950494.1	hypothetical protein	-
AZH48_RS05935	1243755..1243940	+	186	WP_003901093.1	hypothetical protein	-
AZH48_RS05940	1243867..1244142	-	276	WP_003405867.1	hypothetical protein	-
AZH48_RS05945	1244385..1244708	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
AZH48_RS05950	1244723..1245433	-	711	WP_003911399.1	hypothetical protein	-
AZH48_RS22200	1245455..1245664	+	210	WP_003911400.1	hypothetical protein	-
AZH48_RS22205	1245762..1246256	-	495	WP_003405876.1	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T61462 WP_003405865.1 NZ_CP014566:1242362-1242736 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T61462 NZ_CP014566:1242362-1242736 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT61462 WP_003405863.1 NZ_CP014566:1242168-1242365 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT61462 NZ_CP014566:1242168-1242365 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07