Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1233412..1234043 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag AZH48_RS05865 Protein ID WP_003405820.1
Coordinates 1233412..1233723 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag AZH48_RS05870 Protein ID WP_003405836.1
Coordinates 1233723..1234043 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS05845 1228894..1230318 - 1425 WP_003405805.1 class II fumarate hydratase -
AZH48_RS05850 1230349..1231437 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
AZH48_RS05855 1231493..1232137 + 645 WP_174813088.1 DUF4245 domain-containing protein -
AZH48_RS05860 1232144..1233300 - 1157 Protein_1143 AI-2E family transporter -
AZH48_RS05865 1233412..1233723 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
AZH48_RS05870 1233723..1234043 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
AZH48_RS20710 1234053..1235578 + 1526 Protein_1146 carboxylesterase/lipase family protein -
AZH48_RS05885 1235596..1236708 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
AZH48_RS05890 1236718..1236975 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
AZH48_RS05895 1236965..1238212 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
AZH48_RS05900 1238209..1238847 - 639 WP_011799158.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T61461 WP_003405820.1 NZ_CP014566:c1233723-1233412 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T61461 NZ_CP014566:c1233723-1233412 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT61461 WP_003405836.1 NZ_CP014566:c1234043-1233723 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT61461 NZ_CP014566:c1234043-1233723 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References