Detailed information of TA system TA061449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 704490..705133
• Replicon: chromosome
• Accession: NZ_CP014566
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: AZH48_RS03180
• Protein ID: WP_003403187.1
• Coordinates: 704732..705133 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: AZH48_RS03175
• Protein ID: WP_003403184.1
• Coordinates: 704490..704735 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZH48_RS03140	699770..700240	-	471	WP_003403144.1	HAMP domain-containing protein	-
AZH48_RS03145	700284..701045	-	762	WP_010950420.1	two-component system response regulator TcrA	-
AZH48_RS03150	701102..701413	+	312	WP_003403164.1	hypothetical protein	-
AZH48_RS03155	701485..702435	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
AZH48_RS03160	702676..703260	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
AZH48_RS03165	703262..703972	+	711	Protein_627	transposase	-
AZH48_RS03170	704059..704445	+	387	WP_003403180.1	hypothetical protein	-
AZH48_RS03175	704490..704735	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AZH48_RS03180	704732..705133	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
AZH48_RS03185	705076..705303	+	228	WP_003900974.1	hypothetical protein	-
AZH48_RS21695	705521..705688	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
AZH48_RS21700	705721..705918	-	198	WP_003403191.1	hypothetical protein	-
AZH48_RS03195	705998..707155	-	1158	WP_003403193.1	hypothetical protein	-
AZH48_RS03200	707207..707428	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
AZH48_RS03205	707570..708175	+	606	WP_038338260.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T61449 WP_003403187.1 NZ_CP014566:704732-705133 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T61449 NZ_CP014566:704732-705133 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT61449 WP_003403184.1 NZ_CP014566:704490-704735 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT61449 NZ_CP014566:704490-704735 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91