Detailed information of TA system TA061439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 5835321..5836574
• Replicon: chromosome
• Accession: NZ_CP014546
• Organism: Pseudomonas azotoformans strain S4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A127I493
• Locus tag: AYR47_RS26735
• Protein ID: WP_061448990.1
• Coordinates: 5835321..5835911 (-)
• Length: 197 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Y8FYW9
• Locus tag: AYR47_RS26740
• Protein ID: WP_061448991.1
• Coordinates: 5836017..5836574 (-)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR47_RS26715	5830355..5832010	+	1656	WP_061448986.1	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase	-
AYR47_RS26720	5832010..5833530	+	1521	WP_061448987.1	glycosyltransferase family 39 protein	-
AYR47_RS26725	5833540..5834253	-	714	WP_061448988.1	AAA family ATPase	-
AYR47_RS26730	5834253..5834999	-	747	WP_061448989.1	hypothetical protein	-
AYR47_RS26735	5835321..5835911	-	591	WP_061448990.1	HD domain-containing protein	Toxin
AYR47_RS26740	5836017..5836574	-	558	WP_061448991.1	helix-turn-helix domain-containing protein	Antitoxin
AYR47_RS26745	5836638..5837567	-	930	WP_033903316.1	sugar ABC transporter ATP-binding protein	-
AYR47_RS26750	5838252..5838560	+	309	WP_033903317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (9-180)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-48)

Sequences

Toxin

Protein

• Length: 197 a.a.
• Molecular weight: 22096.04 Da
• Isoelectric Point: 5.1549

>T61439 WP_061448990.1 NZ_CP014546:c5835911-5835321 [Pseudomonas azotoformans]
MDTEEIKGRLAFIREAEKLKDVLRSAHTTSGRTESTAEHSWRLCLMAMVFEDQFSHLDRLKLLKMCVIHDLGEAIHGDIP
AIHKASFPDKSEQERKDLLHLTRALDGPLQTQIMALWEDYETAQSAEAKAVKALDKLETLLQHNQGKNPPDFDYGFNLTY
GKGYTDADPLFERLRGLIDEGAREKIAACDSAVLAD

Nucleotide

• Length: 591 bp

>T61439 NZ_CP014546:c5835911-5835321 [Pseudomonas azotoformans]
ATGGACACCGAAGAGATCAAAGGGCGCCTCGCCTTCATTCGCGAGGCGGAAAAACTCAAGGACGTACTCCGCAGCGCCCA
CACCACCTCAGGGCGCACCGAAAGCACCGCGGAACATAGCTGGCGCCTGTGCCTGATGGCGATGGTTTTCGAGGATCAAT
TCAGCCATCTCGACAGGCTCAAACTGCTCAAGATGTGCGTCATTCATGACCTGGGGGAGGCCATCCATGGCGACATCCCG
GCCATCCACAAAGCGTCGTTTCCCGACAAGAGCGAGCAAGAGAGAAAAGACCTTCTACACCTGACCCGCGCCCTCGACGG
GCCATTGCAAACGCAGATAATGGCGCTCTGGGAGGACTATGAAACCGCACAGTCTGCCGAGGCCAAGGCCGTCAAGGCGC
TGGACAAACTAGAGACCCTTCTCCAGCACAATCAGGGCAAGAACCCACCCGATTTCGACTATGGATTCAACCTGACCTAC
GGCAAAGGCTACACGGATGCCGATCCTTTGTTTGAGAGGCTTCGCGGGCTTATTGATGAGGGGGCGCGGGAGAAGATCGC
CGCGTGTGATTCGGCGGTTTTGGCTGATTGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20376.65 Da
• Isoelectric Point: 8.5505

>AT61439 WP_061448991.1 NZ_CP014546:c5836574-5836017 [Pseudomonas azotoformans]
MSDTLLPLALPGVALRRWRLLHRVKQSHAAQVFGVTQSTISRWESGVQAMDPVAHKQLEQLLAARLDAAADHVLARLVSE
SPRPMHLVCDLTHRLLACSPSRAAEFTRPISELMGCSLWRFATPEIMRKELALNASGWRETLAPPAVEFSTGANDSLLVP
ITQGVCRWTRIPLSDGTAARLVETL

Nucleotide

• Length: 558 bp

>AT61439 NZ_CP014546:c5836574-5836017 [Pseudomonas azotoformans]
ATGTCTGACACATTGTTACCGCTGGCCTTGCCTGGCGTTGCGCTGAGGCGCTGGCGCCTGTTGCACCGCGTCAAGCAATC
CCATGCCGCGCAGGTGTTCGGTGTGACCCAATCGACCATTTCCCGCTGGGAAAGCGGCGTGCAGGCCATGGACCCGGTGG
CGCACAAACAGCTTGAGCAACTGCTGGCCGCTCGCCTCGACGCCGCCGCCGATCACGTCCTGGCCCGGCTGGTCAGCGAG
AGCCCACGGCCCATGCACCTTGTCTGCGACCTGACCCACCGTCTGCTGGCGTGCTCCCCGAGCCGAGCCGCCGAGTTCAC
GCGCCCGATCAGTGAGCTGATGGGCTGCTCTCTTTGGCGTTTCGCCACCCCCGAGATCATGCGCAAGGAGCTGGCACTCA
ACGCATCCGGATGGCGCGAAACCCTGGCACCGCCTGCCGTGGAGTTTTCCACGGGCGCCAATGACTCGCTGCTCGTGCCC
ATCACGCAGGGTGTTTGCCGCTGGACCCGCATCCCCCTCTCGGACGGAACGGCCGCGCGCCTGGTCGAGACACTCTGA

Structures

Toxin

• AlphaFold DB: A0A127I493

Antitoxin

• AlphaFold DB: A0A7Y8FYW9