Detailed information of TA system TA061434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3107427..3108031
• Replicon: chromosome
• Accession: NZ_CP014546
• Organism: Pseudomonas azotoformans strain S4

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A127HY59
• Locus tag: AYR47_RS14410
• Protein ID: WP_025999642.1
• Coordinates: 3107738..3108031 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: W2D6C7
• Locus tag: AYR47_RS14405
• Protein ID: WP_033902673.1
• Coordinates: 3107427..3107735 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR47_RS14375	3102730..3103230	-	501	WP_033902678.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
AYR47_RS14380	3103334..3103744	-	411	WP_033902677.1	type II toxin-antitoxin system HicB family antitoxin	-
AYR47_RS14385	3103767..3103949	-	183	WP_012722731.1	type II toxin-antitoxin system HicA family toxin	-
AYR47_RS14390	3104119..3105012	-	894	WP_061435651.1	LysR family transcriptional regulator	-
AYR47_RS14395	3105108..3106271	+	1164	WP_061435653.1	MFS transporter	-
AYR47_RS14400	3106268..3107434	+	1167	WP_061435655.1	MFS transporter	-
AYR47_RS14405	3107427..3107735	-	309	WP_033902673.1	putative addiction module antidote protein	Antitoxin
AYR47_RS14410	3107738..3108031	-	294	WP_025999642.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYR47_RS14420	3108498..3108737	+	240	WP_033902672.1	hypothetical protein	-
AYR47_RS14425	3108816..3109121	-	306	WP_061435657.1	hypothetical protein	-
AYR47_RS14430	3109145..3109669	-	525	WP_061435659.1	DUF3833 domain-containing protein	-
AYR47_RS14440	3110039..3110923	-	885	WP_033902669.1	alpha/beta hydrolase	-
AYR47_RS14445	3111229..3111921	-	693	WP_033902668.1	pseudouridine synthase	-
AYR47_RS14450	3111921..3112133	-	213	WP_033902839.1	cysteine-rich CWC family protein	-
AYR47_RS14455	3112319..3112681	+	363	WP_028619330.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (42-89)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10868.65 Da
• Isoelectric Point: 10.9446

>T61434 WP_025999642.1 NZ_CP014546:c3108031-3107738 [Pseudomonas azotoformans]
VSYLIQQTQAFINWRSEVRDLRAKIAIGRRIERASMGNLGDIKTLGSGISEMRIDVGSGYRIYFTLRGKVIVILLVGGDK
SSQDTDIRRAKKLAKEV

Nucleotide

• Length: 294 bp

>T61434 NZ_CP014546:c3108031-3107738 [Pseudomonas azotoformans]
GTGAGCTATTTGATCCAGCAAACCCAAGCCTTCATTAACTGGCGTTCAGAAGTACGCGACCTACGAGCAAAGATCGCAAT
AGGTCGACGTATCGAAAGAGCCAGCATGGGTAACCTGGGCGATATCAAGACACTCGGTAGCGGCATCTCAGAAATGCGGA
TAGACGTTGGTTCGGGTTATCGAATTTATTTCACTTTGCGAGGTAAGGTGATCGTCATCTTGCTGGTAGGCGGTGACAAG
TCTTCCCAGGACACTGATATCCGTAGAGCAAAAAAATTGGCAAAAGAGGTTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11166.80 Da
• Isoelectric Point: 5.6993

>AT61434 WP_033902673.1 NZ_CP014546:c3107735-3107427 [Pseudomonas azotoformans]
MKQTLTTFDMAELLDSDEAISEYLSQVLADGDNQEFLRAIGYVAKARGMTKIAAETGMGRESLYKAFAPGAKPRFETVLK
VLHSLGIDLFARAGHSVDRSII

Nucleotide

• Length: 309 bp

>AT61434 NZ_CP014546:c3107735-3107427 [Pseudomonas azotoformans]
ATGAAACAAACGCTGACGACATTTGATATGGCCGAATTGCTCGACAGCGACGAGGCCATCAGTGAATACCTCTCTCAGGT
TCTGGCCGACGGCGACAATCAAGAATTTCTTCGCGCCATCGGCTACGTCGCTAAAGCCCGAGGAATGACCAAGATCGCTG
CCGAAACGGGCATGGGTCGTGAGAGCCTTTACAAGGCATTCGCCCCTGGCGCAAAACCTCGCTTCGAAACTGTACTCAAA
GTACTCCACTCCCTCGGTATCGATCTATTTGCGAGGGCTGGACATTCGGTCGACCGCTCGATTATTTGA

Structures

Toxin

• AlphaFold DB: A0A127HY59

Antitoxin

• AlphaFold DB: W2D6C7