Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3103334..3103949 Replicon chromosome
Accession NZ_CP014546
Organism Pseudomonas azotoformans strain S4

Toxin (Protein)


Gene name hicA Uniprot ID W2D6J2
Locus tag AYR47_RS14385 Protein ID WP_012722731.1
Coordinates 3103767..3103949 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID W2D6D2
Locus tag AYR47_RS14380 Protein ID WP_033902677.1
Coordinates 3103334..3103744 (-) Length 137 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR47_RS14340 3098872..3099789 + 918 WP_061435649.1 LysR family transcriptional regulator -
AYR47_RS14345 3099797..3100081 - 285 WP_033902682.1 type II toxin-antitoxin system RelE/ParE family toxin -
AYR47_RS14350 3100071..3100310 - 240 WP_016975132.1 ribbon-helix-helix protein, CopG family -
AYR47_RS14355 3100415..3100825 + 411 WP_033902681.1 fosfomycin resistance glutathione transferase -
AYR47_RS14360 3100880..3101521 + 642 WP_033902680.1 LysE family translocator -
AYR47_RS14365 3101641..3102168 + 528 WP_016975135.1 DUF4142 domain-containing protein -
AYR47_RS14370 3102223..3102648 - 426 WP_038842343.1 low affinity iron permease family protein -
AYR47_RS14375 3102730..3103230 - 501 WP_033902678.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
AYR47_RS14380 3103334..3103744 - 411 WP_033902677.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AYR47_RS14385 3103767..3103949 - 183 WP_012722731.1 type II toxin-antitoxin system HicA family toxin Toxin
AYR47_RS14390 3104119..3105012 - 894 WP_061435651.1 LysR family transcriptional regulator -
AYR47_RS14395 3105108..3106271 + 1164 WP_061435653.1 MFS transporter -
AYR47_RS14400 3106268..3107434 + 1167 WP_061435655.1 MFS transporter -
AYR47_RS14405 3107427..3107735 - 309 WP_033902673.1 putative addiction module antidote protein -
AYR47_RS14410 3107738..3108031 - 294 WP_025999642.1 type II toxin-antitoxin system RelE/ParE family toxin -
AYR47_RS14420 3108498..3108737 + 240 WP_033902672.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3096983..3107926 10943


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 61 a.a.        Molecular weight: 6775.96 Da        Isoelectric Point: 10.7532

>T61433 WP_012722731.1 NZ_CP014546:c3103949-3103767 [Pseudomonas azotoformans]
VDSRYLIGHIVADGWYLARIRGSHHHFKHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Download         Length: 183 bp

>T61433 NZ_CP014546:c3103949-3103767 [Pseudomonas azotoformans]
GTGGATAGCCGATATTTGATAGGTCACATCGTTGCGGACGGTTGGTATCTGGCGCGTATCAGAGGGAGTCATCATCACTT
CAAGCACCCGACTAAACCGGGGCTGGTGACGATTCCACATCCAAAGAAGGACCTGTTGGACAAGACCGCCAAGAGCATTT
TGAAACAAGCTCTGTTGAGTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14721.60 Da        Isoelectric Point: 4.3123

>AT61433 WP_033902677.1 NZ_CP014546:c3103744-3103334 [Pseudomonas azotoformans]
MLYPIAISTGDEDHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQEG

Download         Length: 411 bp

>AT61433 NZ_CP014546:c3103744-3103334 [Pseudomonas azotoformans]
ATGCTTTACCCGATTGCAATTTCAACCGGTGACGAGGATCACGCCTGGGGCGTGGAAGTGCCGGATATTCCTGGTTGCTA
TTCCGCCGGTGACGACCTGGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATCCTGGCCGAAG
ACGGTGCGCCGATTCCCAGTGCGCAGAAGGTCACCCTGCATGCCGCCAACCCCCAGTACGCAGGCTGCACCTGGGCCGTG
GTAGACATTGATGTCACCAAGTATCTGGGCAAGGCGCAGAAGCTCAACATCACGTTGCCGGGTTACCTGCTGAACCGCAT
CGATGAATATGTACTGCACCATCCGGAAGAAAAAAGCCGCTCGGGGTTTCTGGCATCGGCGGCGCTTAAAGTGCTGCAAC
AGGAGGGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB W2D6J2


Antitoxin

Source ID Structure
AlphaFold DB A0A7Y8G830

References