Detailed information of TA system TA061432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 3099797..3100310
• Replicon: chromosome
• Accession: NZ_CP014546
• Organism: Pseudomonas azotoformans strain S4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W2D845
• Locus tag: AYR47_RS14345
• Protein ID: WP_033902682.1
• Coordinates: 3099797..3100081 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W2D6D8
• Locus tag: AYR47_RS14350
• Protein ID: WP_016975132.1
• Coordinates: 3100071..3100310 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR47_RS14325	3095982..3096923	+	942	WP_061435645.1	hydroxymethylglutaryl-CoA lyase	-
AYR47_RS14330	3096983..3098314	+	1332	WP_061435646.1	MFS transporter	-
AYR47_RS14335	3098315..3098803	-	489	WP_061435647.1	DMT family transporter	-
AYR47_RS14340	3098872..3099789	+	918	WP_061435649.1	LysR family transcriptional regulator	-
AYR47_RS14345	3099797..3100081	-	285	WP_033902682.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYR47_RS14350	3100071..3100310	-	240	WP_016975132.1	ribbon-helix-helix protein, CopG family	Antitoxin
AYR47_RS14355	3100415..3100825	+	411	WP_033902681.1	fosfomycin resistance glutathione transferase	-
AYR47_RS14360	3100880..3101521	+	642	WP_033902680.1	LysE family translocator	-
AYR47_RS14365	3101641..3102168	+	528	WP_016975135.1	DUF4142 domain-containing protein	-
AYR47_RS14370	3102223..3102648	-	426	WP_038842343.1	low affinity iron permease family protein	-
AYR47_RS14375	3102730..3103230	-	501	WP_033902678.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
AYR47_RS14380	3103334..3103744	-	411	WP_033902677.1	type II toxin-antitoxin system HicB family antitoxin	-
AYR47_RS14385	3103767..3103949	-	183	WP_012722731.1	type II toxin-antitoxin system HicA family toxin	-
AYR47_RS14390	3104119..3105012	-	894	WP_061435651.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3096983..3107926	10943

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-83)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10939.64 Da
• Isoelectric Point: 10.1506

>T61432 WP_033902682.1 NZ_CP014546:c3100081-3099797 [Pseudomonas azotoformans]
MQVEWLRAALRNLDDEAAYIAEENPKAAHEFVQAILTGLQQIAEFPAMGKEGRVPGTREWVVPRWPYIVPYRIRGGRLQV
MRIFHTRRQPPASW

Nucleotide

• Length: 285 bp

>T61432 NZ_CP014546:c3100081-3099797 [Pseudomonas azotoformans]
ATGCAGGTTGAGTGGCTGAGGGCAGCCCTCAGGAACCTTGACGATGAAGCCGCTTATATTGCTGAAGAAAATCCAAAAGC
TGCCCACGAATTTGTCCAGGCAATCTTGACTGGACTCCAACAGATTGCTGAATTTCCAGCGATGGGAAAAGAGGGACGCG
TGCCAGGGACTCGCGAATGGGTAGTGCCTAGATGGCCCTACATTGTCCCGTATCGCATCCGAGGGGGCCGATTGCAAGTC
ATGCGGATCTTCCATACCCGGCGCCAGCCACCCGCATCCTGGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8612.78 Da
• Isoelectric Point: 4.7719

>AT61432 WP_016975132.1 NZ_CP014546:c3100310-3100071 [Pseudomonas azotoformans]
MSVMSLRIPEDIADTLASLAKATGRSKSFLAVDALREYLTREAWQIEEIQKALKEADAGDFATAAEVKAVADKWRTNAG

Nucleotide

• Length: 240 bp

>AT61432 NZ_CP014546:c3100310-3100071 [Pseudomonas azotoformans]
ATGTCTGTCATGTCCCTACGTATACCGGAAGATATCGCCGATACGCTTGCCAGTCTCGCCAAGGCCACCGGGCGCAGCAA
ATCCTTTCTGGCGGTAGACGCATTGCGCGAATACCTCACGCGCGAAGCCTGGCAAATTGAGGAAATTCAAAAAGCATTGA
AAGAAGCGGATGCGGGTGATTTCGCTACCGCTGCCGAGGTAAAAGCTGTCGCTGATAAATGGCGCACTAATGCAGGTTGA

Structures

Toxin

• AlphaFold DB: A0A7Y8KKJ0

Antitoxin

• AlphaFold DB: W2D6D8