Detailed information of TA system TA061431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 2858807..2859377
• Replicon: chromosome
• Accession: NZ_CP014546
• Organism: Pseudomonas azotoformans strain S4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AYR47_RS13220
• Protein ID: WP_061435497.1
• Coordinates: 2858807..2859010 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W2DU62
• Locus tag: AYR47_RS13225
• Protein ID: WP_033899935.1
• Coordinates: 2859087..2859377 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR47_RS13195	2854639..2855334	-	696	WP_061435493.1	tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO	-
AYR47_RS13200	2855404..2855865	-	462	WP_032893248.1	DUF1456 family protein	-
AYR47_RS13205	2855936..2856647	-	712	Protein_2631	rRNA pseudouridine synthase	-
AYR47_RS13210	2856719..2857192	-	474	WP_061435495.1	GNAT family N-acetyltransferase	-
AYR47_RS13215	2857297..2858625	-	1329	WP_169855583.1	30S ribosomal protein S12 methylthiotransferase RimO	-
AYR47_RS13220	2858807..2859010	-	204	WP_061435497.1	type II toxin-antitoxin system YafQ family toxin	Toxin
AYR47_RS13225	2859087..2859377	-	291	WP_033899935.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AYR47_RS13230	2859759..2861661	+	1903	Protein_2636	potassium transporter Kup	-
AYR47_RS13235	2861789..2862895	+	1107	WP_033899937.1	NADH:flavin oxidoreductase/NADH oxidase	-
AYR47_RS13240	2863000..2863545	+	546	WP_061435499.1	GNAT family N-acetyltransferase	-
AYR47_RS13245	2863740..2863925	+	186	WP_033899941.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-66)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (10-84)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 7706.78 Da
• Isoelectric Point: 4.2819

>T61431 WP_061435497.1 NZ_CP014546:c2859010-2858807 [Pseudomonas azotoformans]
MNEVRVVMVMLFLGEQLPAEYRDHALTGNWDGFRECHIGGDFLMIYENSRVDLITFVDLGSHAELFS

Nucleotide

• Length: 204 bp

>T61431 NZ_CP014546:c2859010-2858807 [Pseudomonas azotoformans]
ATGAATGAGGTCCGGGTAGTGATGGTGATGCTTTTCTTAGGCGAGCAACTACCCGCGGAGTATCGCGACCACGCGCTTAC
CGGGAATTGGGACGGTTTCAGGGAGTGCCATATCGGTGGTGATTTCCTGATGATTTACGAGAACTCGCGGGTTGACCTGA
TTACCTTCGTGGATTTGGGGAGCCATGCAGAATTGTTCTCGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10759.28 Da
• Isoelectric Point: 6.3340

>AT61431 WP_033899935.1 NZ_CP014546:c2859377-2859087 [Pseudomonas azotoformans]
MSTLLKTTDVRCRIDEDLKERATAVLNACGLSLSDAMRLFLRQVVTTQGLPFEVRVPSEKTARAMTQARSIRRQFDSIDE
MLRDADGEAGEEAKAR

Nucleotide

• Length: 291 bp

>AT61431 NZ_CP014546:c2859377-2859087 [Pseudomonas azotoformans]
ATGTCCACATTACTGAAAACCACTGACGTGCGTTGCCGCATCGACGAGGATTTGAAGGAGCGTGCCACCGCTGTGCTCAA
TGCTTGTGGGCTGAGCCTCAGTGACGCCATGCGCCTTTTCCTGCGTCAGGTCGTAACAACGCAGGGATTACCCTTTGAGG
TGCGCGTCCCTTCGGAGAAGACCGCTCGTGCGATGACACAAGCGCGTTCCATTCGTCGCCAGTTTGACTCGATCGACGAG
ATGCTAAGGGACGCTGATGGCGAAGCCGGAGAAGAAGCAAAAGCGCGCTGA

Structures

Antitoxin

• AlphaFold DB: W2DU62