Detailed information of TA system TA061427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 312972..313477
• Replicon: chromosome
• Accession: NZ_CP014546
• Organism: Pseudomonas azotoformans strain S4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W2E338
• Locus tag: AYR47_RS01505
• Protein ID: WP_033896222.1
• Coordinates: 313223..313477 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: W2E3T5
• Locus tag: AYR47_RS01500
• Protein ID: WP_033896223.1
• Coordinates: 312972..313226 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR47_RS01485	308488..309387	+	900	WP_061434020.1	LysR family transcriptional regulator	-
AYR47_RS01490	309471..310721	+	1251	WP_061434022.1	aspartate aminotransferase family protein	-
AYR47_RS01495	310718..312742	-	2025	WP_061434024.1	c-type cytochrome	-
AYR47_RS01500	312972..313226	+	255	WP_033896223.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
AYR47_RS01505	313223..313477	+	255	WP_033896222.1	Txe/YoeB family addiction module toxin	Toxin
AYR47_RS01510	313547..314050	+	504	WP_061434026.1	hypothetical protein	-
AYR47_RS01515	314121..314843	+	723	WP_033896220.1	hypothetical protein	-
AYR47_RS01520	314891..316090	-	1200	WP_208603924.1	MFS transporter	-
AYR47_RS01525	316265..316957	-	693	WP_061434030.1	LuxR family transcriptional regulator	-
AYR47_RS01530	317100..318191	-	1092	WP_061434032.1	pyridoxal phosphate-dependent aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10049.57 Da
• Isoelectric Point: 7.2754

>T61427 WP_033896222.1 NZ_CP014546:313223-313477 [Pseudomonas azotoformans]
MKILFTPEGWDDYLWFQQNDKAGLKRINLLIKAIQRDPFEGVGKPEPLKHNLSGFWSRRITAEHRLVYGIEDDEVQILMC
RYHY

Nucleotide

• Length: 255 bp

>T61427 NZ_CP014546:313223-313477 [Pseudomonas azotoformans]
ATGAAAATACTCTTTACCCCTGAGGGCTGGGACGACTATCTGTGGTTTCAACAGAACGACAAAGCAGGGCTCAAGCGTAT
AAACCTGCTGATCAAAGCCATCCAGCGGGATCCATTCGAGGGCGTTGGCAAGCCTGAGCCGCTCAAACACAACCTGAGTG
GTTTCTGGTCTCGACGGATCACTGCAGAGCATCGTCTGGTTTATGGTATTGAAGACGACGAAGTGCAGATACTGATGTGC
CGTTATCACTACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9588.99 Da
• Isoelectric Point: 7.2037

>AT61427 WP_033896223.1 NZ_CP014546:312972-313226 [Pseudomonas azotoformans]
METINYTNARAHLAETMDRVNEDHIPLLLTRQKGEPVVMISLSEFNALEETAYLLRSPKNAERLINSVNSLRAGKAKPRR
LIEE

Nucleotide

• Length: 255 bp

>AT61427 NZ_CP014546:312972-313226 [Pseudomonas azotoformans]
ATGGAAACTATCAACTACACCAATGCCCGCGCTCATCTGGCCGAAACAATGGACCGGGTTAACGAAGATCACATTCCACT
GCTACTGACCCGGCAAAAGGGTGAGCCGGTGGTGATGATTTCACTGTCAGAATTCAATGCCCTTGAAGAGACTGCCTATC
TGCTGCGCTCGCCTAAAAATGCCGAACGCCTCATCAACTCAGTCAACAGCCTGAGAGCCGGTAAAGCGAAGCCAAGGCGG
TTAATTGAAGAATGA

Structures

Toxin

• AlphaFold DB: W2E338

Antitoxin

• AlphaFold DB: W2E3T5