Detailed information of TA system TA061410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 20095..20620
• Replicon: plasmid pSO3_STV
• Accession: NZ_CP014537
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SO3 isolate SOHS 02-68

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: SE18cs_RS23980
• Protein ID: WP_001159863.1
• Coordinates: 20315..20620 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: SE18cs_RS23975
• Protein ID: WP_000813641.1
• Coordinates: 20095..20313 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SE18cs_RS23950	15382..15763	+	382	Protein_20	YgiW/YdeI family stress tolerance OB fold protein	-
SE18cs_RS26590	15932..16376	+	445	Protein_21	site-specific integrase	-
SE18cs_RS23960	16635..17624	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
SE18cs_RS26310	18118..18414	-	297	WP_001687482.1	hypothetical protein	-
SE18cs_RS23970	18898..19419	-	522	WP_010999942.1	hypothetical protein	-
SE18cs_RS23975	20095..20313	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
SE18cs_RS23980	20315..20620	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
SE18cs_RS23985	20622..20912	+	291	WP_001266176.1	hypothetical protein	-
SE18cs_RS23990	20909..21430	+	522	WP_000198608.1	hypothetical protein	-
SE18cs_RS23995	21465..22247	+	783	WP_000082169.1	site-specific integrase	-
SE18cs_RS24000	22256..22969	+	714	Protein_30	EAL domain-containing protein	-
SE18cs_RS24005	22973..23482	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
SE18cs_RS24010	23476..23961	+	486	WP_000905606.1	membrane protein	-
SE18cs_RS26595	24238..24525	-	288	WP_071530243.1	hypothetical protein	-
SE18cs_RS24020	24681..25241	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93844	93844

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T61410 WP_001159863.1 NZ_CP014537:20315-20620 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T61410 NZ_CP014537:20315-20620 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT61410 WP_000813641.1 NZ_CP014537:20095-20313 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT61410 NZ_CP014537:20095-20313 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6