Detailed information of TA system TA061409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 12838..13445
• Replicon: plasmid pl5
• Accession: NZ_CP014534
• Organism: Enterococcus faecium strain E745

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: EfmE745_RS15115
• Protein ID: WP_002321032.1
• Coordinates: 13095..13445 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: EfmE745_RS15110
• Protein ID: WP_002287514.1
• Coordinates: 12838..13101 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EfmE745_RS15065	7874..8773	+	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
EfmE745_RS15070	8776..9261	+	486	WP_002296241.1	single-stranded DNA-binding protein	-
EfmE745_RS15075	9627..9887	+	261	WP_002296240.1	hypothetical protein	-
EfmE745_RS15080	10328..10534	+	207	WP_002322893.1	hypothetical protein	-
EfmE745_RS15085	10534..10785	+	252	WP_002296238.1	hypothetical protein	-
EfmE745_RS15090	10800..11237	+	438	WP_002296237.1	hypothetical protein	-
EfmE745_RS15095	11230..11937	+	708	WP_002323031.1	hypothetical protein	-
EfmE745_RS15105	12318..12497	+	180	WP_002305761.1	hypothetical protein	-
EfmE745_RS15110	12838..13101	+	264	WP_002287514.1	hypothetical protein	Antitoxin
EfmE745_RS15115	13095..13445	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EfmE745_RS15120	13469..14083	-	615	WP_002318230.1	recombinase family protein	-
EfmE745_RS15125	14533..15858	+	1326	WP_002319870.1	Y-family DNA polymerase	-
EfmE745_RS15130	15851..16201	+	351	WP_002323028.1	hypothetical protein	-
EfmE745_RS15135	16417..16710	+	294	WP_002326890.1	hypothetical protein	-
EfmE745_RS15140	17030..17872	+	843	WP_002323027.1	ParA family protein	-
EfmE745_RS15145	17875..18429	+	555	WP_002323026.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55167	55167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T61409 WP_002321032.1 NZ_CP014534:13095-13445 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T61409 NZ_CP014534:13095-13445 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT61409 WP_002287514.1 NZ_CP014534:12838-13101 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT61409 NZ_CP014534:12838-13101 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0