Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 3990..4509 Replicon plasmid pl4
Accession NZ_CP014533
Organism Enterococcus faecium strain E745

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag EfmE745_RS14945 Protein ID WP_000588503.1
Coordinates 4252..4509 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag EfmE745_RS14940 Protein ID WP_000388479.1
Coordinates 3990..4259 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EfmE745_RS14915 524..1564 + 1041 WP_000997689.1 replication initiator protein A -
EfmE745_RS14920 1861..2542 - 682 Protein_3 IS6 family transposase -
EfmE745_RS14925 2604..3299 + 696 WP_002326819.1 hypothetical protein -
EfmE745_RS14935 3680..3859 + 180 WP_001120991.1 hypothetical protein -
EfmE745_RS14940 3990..4259 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EfmE745_RS14945 4252..4509 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
EfmE745_RS14950 4968..5972 + 1005 WP_000455809.1 hypothetical protein -
EfmE745_RS14955 6137..6751 - 615 WP_001261742.1 recombinase family protein -
EfmE745_RS14960 7201..8525 + 1325 Protein_10 Y-family DNA polymerase -
EfmE745_RS14965 8518..8866 + 349 Protein_11 hypothetical protein -
EfmE745_RS14970 8863..9042 + 180 WP_001021554.1 hypothetical protein -
EfmE745_RS14975 9179..9469 + 291 WP_000026576.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..17254 17254
- flank IS/Tn - - 1892..2548 656


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T61408 WP_000588503.1 NZ_CP014533:4252-4509 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T61408 NZ_CP014533:4252-4509 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT61408 WP_000388479.1 NZ_CP014533:3990-4259 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT61408 NZ_CP014533:3990-4259 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References