Detailed information of TA system TA061401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 12108..12652
• Replicon: plasmid unnamed 1
• Accession: NZ_CP014526
• Organism: Haematospirillum jordaniae strain H5569

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A143DGE5
• Locus tag: AY555_RS10055
• Protein ID: WP_066136937.1
• Coordinates: 12108..12431 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A143DG76
• Locus tag: AY555_RS10060
• Protein ID: WP_066136939.1
• Coordinates: 12428..12652 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AY555_RS10030	7461..8255	-	795	WP_066136930.1	GPO family capsid scaffolding protein	-
AY555_RS10035	8399..10174	+	1776	WP_066136932.1	terminase family protein	-
AY555_RS10040	10174..11118	+	945	WP_066136933.1	phage portal protein	-
AY555_RS10045	11180..11650	-	471	WP_066136935.1	type II toxin-antitoxin system HicB family antitoxin	-
AY555_RS10050	11666..11893	-	228	WP_066136992.1	type II toxin-antitoxin system HicA family toxin	-
AY555_RS10055	12108..12431	-	324	WP_066136937.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AY555_RS10060	12428..12652	-	225	WP_066136939.1	antitoxin MazE family protein	Antitoxin
AY555_RS10065	12850..13104	+	255	WP_066136941.1	type II toxin-antitoxin system HicA family toxin	-
AY555_RS10070	13101..13529	+	429	WP_082812125.1	type II toxin-antitoxin system HicB family antitoxin	-
AY555_RS10075	13933..14178	-	246	WP_066136943.1	hypothetical protein	-
AY555_RS10080	14144..14803	-	660	WP_066136944.1	AAA family ATPase	-
AY555_RS10085	15354..15743	+	390	WP_209315880.1	TIR domain-containing protein	-
AY555_RS10090	15698..16249	+	552	WP_156483395.1	hypothetical protein	-
AY555_RS10095	16456..17010	+	555	WP_066136995.1	recombinase family protein	-
AY555_RS11635	17017..17544	-	528	WP_082812127.1	BrnA antitoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32967	32967
	-	flank	IS/Tn	-	-	16426..17010	584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (8-71)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11717.75 Da
• Isoelectric Point: 8.7847

>T61401 WP_066136937.1 NZ_CP014526:c12431-12108 [Haematospirillum jordaniae]
MRRGDLVTISLPGDFGKPRPALVIQSDQFIETATVSVLLMTGTLVDAPLIRMDIVPDTDNGLRKPSQIMVDKVMTVRRDK
VGEAFGRLDDKTMVSVNRALALFLGFG

Nucleotide

• Length: 324 bp

>T61401 NZ_CP014526:c12431-12108 [Haematospirillum jordaniae]
TTGAGGCGCGGTGATCTTGTGACTATCTCCTTGCCGGGCGACTTCGGGAAGCCGCGCCCGGCCTTGGTGATCCAATCCGA
TCAGTTTATTGAGACGGCAACCGTCTCGGTTCTGCTGATGACAGGCACATTGGTTGATGCACCGCTGATCCGCATGGATA
TTGTTCCGGATACTGACAACGGGCTGCGCAAGCCCTCACAGATCATGGTCGACAAGGTTATGACCGTGCGTCGTGACAAG
GTGGGCGAGGCTTTTGGCAGGCTGGATGATAAAACCATGGTCAGCGTGAATAGGGCTTTGGCGCTGTTCCTCGGGTTCGG
GTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8271.36 Da
• Isoelectric Point: 6.9773

>AT61401 WP_066136939.1 NZ_CP014526:c12652-12428 [Haematospirillum jordaniae]
MPTPVSGRVNKRRAALRAAGLRPVQIWIPDTRQPGFREECQRQAHVVARADRADAGLYDFMDAALLDLDDEAQP

Nucleotide

• Length: 225 bp

>AT61401 NZ_CP014526:c12652-12428 [Haematospirillum jordaniae]
ATGCCAACGCCAGTCTCTGGGCGGGTAAACAAGCGCCGGGCTGCTCTGAGGGCTGCGGGACTCCGGCCAGTCCAGATATG
GATTCCTGATACCCGCCAACCGGGTTTCAGAGAGGAATGCCAGCGCCAAGCCCATGTTGTGGCTCGCGCGGACAGGGCAG
ACGCTGGTTTGTATGACTTTATGGACGCGGCTCTTCTCGATCTGGACGATGAGGCGCAACCTTGA

Structures

Toxin

• AlphaFold DB: A0A143DGE5

Antitoxin

• AlphaFold DB: A0A143DG76