Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 64523..65048 Replicon plasmid pZH063_1
Accession NZ_CP014523
Organism Escherichia coli strain ZH063

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag AVR76_RS25505 Protein ID WP_001159871.1
Coordinates 64523..64828 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AVR76_RS25510 Protein ID WP_000813634.1
Coordinates 64830..65048 (-) Length 73 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR76_RS25475 59790..61060 + 1271 Protein_76 translesion error-prone DNA polymerase V subunit UmuC -
AVR76_RS25480 61065..61457 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
AVR76_RS25485 61462..62433 - 972 WP_001103694.1 hypothetical protein -
AVR76_RS25490 62662..63306 + 645 WP_000633911.1 AAA family ATPase -
AVR76_RS25495 63300..63575 + 276 WP_000239529.1 hypothetical protein -
AVR76_RS25500 63713..64522 - 810 WP_000016979.1 site-specific integrase -
AVR76_RS25505 64523..64828 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
AVR76_RS25510 64830..65048 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AVR76_RS25515 67370..68011 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114223 114223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-100)

Antitoxin

(2-72)


Sequences


Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T61400 WP_001159871.1 NZ_CP014523:c64828-64523 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T61400 NZ_CP014523:c64828-64523 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT61400 WP_000813634.1 NZ_CP014523:c65048-64830 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT61400 NZ_CP014523:c65048-64830 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CCE8


Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References