Detailed information of TA system TA061399

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4899552..4900253
• Replicon: chromosome
• Accession: NZ_CP014522
• Organism: Escherichia coli strain ZH063

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A515RBV3
• Locus tag: AVR76_RS24435
• Protein ID: WP_001531265.1
• Coordinates: 4899918..4900253 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AVR76_RS24430
• Protein ID: WP_032143152.1
• Coordinates: 4899552..4899887 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR76_RS24405	4895499..4895717	-	219	WP_001531256.1	AlpA family phage regulatory protein	-
AVR76_RS24410	4895835..4896449	-	615	WP_001531257.1	inovirus Gp2 family protein	-
AVR76_RS24415	4897042..4897995	+	954	WP_001531259.1	hypothetical protein	-
AVR76_RS24420	4898231..4899049	+	819	WP_001531260.1	DUF945 domain-containing protein	-
AVR76_RS24425	4899079..4899552	+	474	WP_001531261.1	DNA repair protein RadC	-
AVR76_RS24430	4899552..4899887	+	336	WP_032143152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AVR76_RS24435	4899918..4900253	+	336	WP_001531265.1	TA system toxin CbtA family protein	Toxin
AVR76_RS24440	4900384..4901217	+	834	WP_001531266.1	DUF4942 domain-containing protein	-
AVR76_RS24445	4901294..4901542	+	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
AVR76_RS24450	4901546..4901821	+	276	WP_001531268.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AVR76_RS24455	4901935..4902732	+	798	WP_001531269.1	helix-turn-helix transcriptional regulator	-
AVR76_RS28515	4903143..4903314	+	172	Protein_4649	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (11-110)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12867.89 Da
• Isoelectric Point: 9.8953

>T61399 WP_001531265.1 NZ_CP014522:4899918-4900253 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRRHNTP

Nucleotide

• Length: 336 bp

>T61399 NZ_CP014522:4899918-4900253 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACTACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCCG
CCATAACACGCCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.48 Da
• Isoelectric Point: 7.1637

>AT61399 WP_032143152.1 NZ_CP014522:4899552-4899887 [Escherichia coli]
MLQKDKVMQQECGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADDELSACQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT61399 NZ_CP014522:4899552-4899887 [Escherichia coli]
ATGCTGCAAAAGGATAAGGTCATGCAACAAGAATGTGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGATGAACTCTCCGCCTGCCAACAACGT
ACCGTCACACTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A515RBV3