Detailed information of TA system TA061396

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4160113..4160948
• Replicon: chromosome
• Accession: NZ_CP014522
• Organism: Escherichia coli strain ZH063

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A376WVB1
• Locus tag: AVR76_RS20900
• Protein ID: WP_001094448.1
• Coordinates: 4160571..4160948 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3T7EC32
• Locus tag: AVR76_RS20895
• Protein ID: WP_024175935.1
• Coordinates: 4160113..4160481 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR76_RS20870	4157542..4157775	+	234	WP_001117568.1	DUF905 family protein	-
AVR76_RS20875	4157866..4158684	+	819	WP_001234753.1	DUF945 domain-containing protein	-
AVR76_RS20880	4158776..4159261	+	486	WP_000214415.1	antirestriction protein	-
AVR76_RS20885	4159273..4159749	+	477	WP_001618155.1	RadC family protein	-
AVR76_RS20890	4159818..4160039	+	222	WP_000692346.1	DUF987 domain-containing protein	-
AVR76_RS20895	4160113..4160481	+	369	WP_024175935.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AVR76_RS20900	4160571..4160948	+	378	WP_001094448.1	TA system toxin CbtA family protein	Toxin
AVR76_RS20905	4160945..4161433	+	489	WP_000761714.1	hypothetical protein	-
AVR76_RS20910	4161445..4161666	+	222	WP_001296561.1	DUF957 domain-containing protein	-
AVR76_RS20915	4161722..4162567	+	846	WP_001576423.1	DUF4942 domain-containing protein	-
AVR76_RS20925	4163160..4163657	+	498	WP_000509815.1	hypothetical protein	-
AVR76_RS20930	4163835..4164758	+	924	WP_000535950.1	carboxylate/amino acid/amine transporter	-
AVR76_RS20935	4164762..4165580	-	819	WP_000779409.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4152378..4163657	11279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14221.22 Da
• Isoelectric Point: 8.2919

>T61396 WP_001094448.1 NZ_CP014522:4160571-4160948 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T61396 NZ_CP014522:4160571-4160948 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13598.40 Da
• Isoelectric Point: 6.6255

>AT61396 WP_024175935.1 NZ_CP014522:4160113-4160481 [Escherichia coli]
VSDTLSGTTHPDDNDDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT61396 NZ_CP014522:4160113-4160481 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A376WVB1

Antitoxin

• AlphaFold DB: A0A3T7EC32