Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3185789..3186443 Replicon chromosome
Accession NZ_CP014522
Organism Escherichia coli strain ZH063

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AVR76_RS16010 Protein ID WP_000244781.1
Coordinates 3185789..3186196 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AVR76_RS16015 Protein ID WP_000354046.1
Coordinates 3186177..3186443 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR76_RS15990 3181746..3183479 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
AVR76_RS15995 3183485..3184195 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AVR76_RS16000 3184220..3185116 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AVR76_RS16005 3185228..3185749 + 522 WP_001055874.1 flavodoxin FldB -
AVR76_RS16010 3185789..3186196 - 408 WP_000244781.1 protein YgfX Toxin
AVR76_RS16015 3186177..3186443 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AVR76_RS16020 3186686..3187666 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
AVR76_RS16025 3187743..3188402 - 660 WP_000250274.1 hemolysin III family protein -
AVR76_RS16030 3188566..3188877 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AVR76_RS16035 3188922..3190355 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T61391 WP_000244781.1 NZ_CP014522:c3186196-3185789 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T61391 NZ_CP014522:c3186196-3185789 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT61391 WP_000354046.1 NZ_CP014522:c3186443-3186177 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT61391 NZ_CP014522:c3186443-3186177 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References