Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2212117..2212948 Replicon chromosome
Accession NZ_CP014522
Organism Escherichia coli strain ZH063

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag AVR76_RS11425 Protein ID WP_000854815.1
Coordinates 2212574..2212948 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag AVR76_RS11420 Protein ID WP_001280918.1
Coordinates 2212117..2212485 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR76_RS11380 2207708..2208385 + 678 WP_001362823.1 hypothetical protein -
AVR76_RS11385 2208401..2208811 + 411 WP_000846703.1 hypothetical protein -
AVR76_RS11390 2209032..2209850 + 819 WP_001542275.1 DUF945 domain-containing protein -
AVR76_RS11395 2209850..2210095 + 246 WP_001164966.1 hypothetical protein -
AVR76_RS11400 2210189..2210662 + 474 WP_001542276.1 antirestriction protein -
AVR76_RS11405 2210678..2211154 + 477 WP_001186200.1 RadC family protein -
AVR76_RS11410 2211217..2211438 + 222 WP_000692345.1 DUF987 domain-containing protein -
AVR76_RS11415 2211457..2212101 + 645 WP_000086752.1 hypothetical protein -
AVR76_RS11420 2212117..2212485 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AVR76_RS11425 2212574..2212948 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
AVR76_RS11430 2212945..2213139 + 195 WP_000988600.1 hypothetical protein -
AVR76_RS28875 2213152..2213265 + 114 WP_001161660.1 DUF957 domain-containing protein -
AVR76_RS28880 2213554..2213700 - 147 Protein_2189 transposase domain-containing protein -
AVR76_RS28885 2213754..2213936 + 183 WP_001296208.1 hypothetical protein -
AVR76_RS11445 2214037..2214366 - 330 WP_000450409.1 DUF496 family protein -
AVR76_RS11450 2214538..2215596 - 1059 WP_001200889.1 FUSC family protein -
AVR76_RS11455 2215794..2216267 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
AVR76_RS11460 2216386..2217552 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T61389 WP_000854815.1 NZ_CP014522:2212574-2212948 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T61389 NZ_CP014522:2212574-2212948 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT61389 WP_001280918.1 NZ_CP014522:2212117-2212485 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT61389 NZ_CP014522:2212117-2212485 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References