Detailed information of TA system TA061362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1101435..1102033
• Replicon: chromosome
• Accession: NZ_CP014505
• Organism: Burkholderia sp. PAMC 28687

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0X8L7F3
• Locus tag: AX768_RS05060
• Protein ID: WP_061999041.1
• Coordinates: 1101725..1102033 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AX768_RS05055
• Protein ID: WP_061999040.1
• Coordinates: 1101435..1101728 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX768_RS05040	1097807..1098952	+	1146	WP_061999037.1	PAS domain-containing sensor histidine kinase	-
AX768_RS05045	1099098..1100597	+	1500	WP_061999038.1	nitrogen regulation protein NR(I)	-
AX768_RS05050	1100631..1101407	-	777	WP_061999039.1	exodeoxyribonuclease III	-
AX768_RS05055	1101435..1101728	-	294	WP_061999040.1	putative addiction module antidote protein	Antitoxin
AX768_RS05060	1101725..1102033	-	309	WP_061999041.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AX768_RS05065	1102270..1103544	+	1275	WP_061999042.1	amidohydrolase family protein	-
AX768_RS05070	1103606..1105738	-	2133	WP_061999043.1	M3 family metallopeptidase	-
AX768_RS05075	1105925..1106788	-	864	WP_061999044.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11559.34 Da
• Isoelectric Point: 10.5091

>T61362 WP_061999041.1 NZ_CP014505:c1102033-1101725 [Burkholderia sp. PAMC 28687]
MTNTINQTREFSGWLLSVKDLSARATILVRIKRAAKGNFGDCHDVGESVWEMRIHTGAGYRVYYVRDGLSVYLLLAGGCK
QGQVADIARAKAMWQRIRQERK

Nucleotide

• Length: 309 bp

>T61362 NZ_CP014505:c1102033-1101725 [Burkholderia sp. PAMC 28687]
ATGACCAACACCATCAATCAAACGCGTGAATTTTCAGGTTGGCTGTTGAGCGTAAAAGACCTGAGCGCACGTGCCACGAT
CCTGGTCCGGATCAAGCGCGCAGCGAAGGGTAATTTTGGCGATTGCCACGATGTTGGCGAAAGCGTCTGGGAAATGCGGA
TCCACACCGGCGCGGGGTATCGTGTTTACTATGTGCGCGACGGCCTGAGCGTCTATCTCTTGTTAGCAGGCGGCTGCAAG
CAAGGGCAAGTAGCGGATATCGCCCGAGCGAAGGCCATGTGGCAGCGAATCAGACAGGAACGGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10194.52 Da
• Isoelectric Point: 4.7483

>AT61362 WP_061999040.1 NZ_CP014505:c1101728-1101435 [Burkholderia sp. PAMC 28687]
MNSTQISAFDASDYLDSEEAIAAYLNEALASGDSDILLSALADVVKARGMSKVASDAGVQRESLYKSLSEGAKPRFETIQ
KVAAALGVKLVAVPLHH

Nucleotide

• Length: 294 bp

>AT61362 NZ_CP014505:c1101728-1101435 [Burkholderia sp. PAMC 28687]
ATGAACTCCACTCAAATTTCAGCATTCGACGCATCCGACTATCTCGACAGCGAAGAGGCCATCGCAGCTTATCTGAATGA
AGCGCTTGCTTCGGGCGACTCGGATATTCTGCTCTCGGCGCTTGCCGACGTCGTAAAGGCACGCGGCATGTCGAAGGTCG
CCTCCGACGCCGGCGTGCAGCGCGAAAGCCTTTACAAGTCGCTCTCCGAAGGCGCGAAACCCCGGTTCGAAACCATCCAG
AAAGTAGCGGCCGCGCTGGGCGTCAAACTCGTCGCCGTGCCGCTGCATCACTGA

Structures

Toxin

• AlphaFold DB: A0A0X8L7F3