Detailed information of TA system TA061361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 286275..286839
• Replicon: chromosome
• Accession: NZ_CP014505
• Organism: Burkholderia sp. PAMC 28687

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: AX768_RS01385
• Protein ID: WP_061998462.1
• Coordinates: 286561..286839 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A242M622
• Locus tag: AX768_RS01380
• Protein ID: WP_061998461.1
• Coordinates: 286275..286532 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX768_RS01355	281696..282474	-	779	Protein_268	paraquat-inducible protein A	-
AX768_RS01360	282905..283465	+	561	WP_061998457.1	cytochrome b	-
AX768_RS01365	283623..284195	+	573	WP_061998458.1	YceI family protein	-
AX768_RS01370	284240..284824	+	585	WP_061998459.1	YceI family protein	-
AX768_RS01375	284980..286254	+	1275	WP_061998460.1	MFS transporter	-
AX768_RS01380	286275..286532	-	258	WP_061998461.1	BrnA antitoxin family protein	Antitoxin
AX768_RS01385	286561..286839	-	279	WP_061998462.1	BrnT family toxin	Toxin
AX768_RS01390	287024..287974	-	951	WP_061998463.1	protein translocase subunit SecF	-
AX768_RS01395	287992..290061	-	2070	WP_061998464.1	protein translocase subunit SecD	-
AX768_RS01400	290182..290505	-	324	WP_061998465.1	preprotein translocase subunit YajC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-77)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10802.30 Da
• Isoelectric Point: 7.2046

>T61361 WP_061998462.1 NZ_CP014505:c286839-286561 [Burkholderia sp. PAMC 28687]
MRFEWDEAKNRSNVRKHGVDFNDAIEIFKHPVLTAADRREDFDEDRWIALGWMKLIVALAVYVERDGDIVRIISARKATR
PEIKRYGDAISQ

Nucleotide

• Length: 279 bp

>T61361 NZ_CP014505:c286839-286561 [Burkholderia sp. PAMC 28687]
ATGCGATTCGAGTGGGATGAAGCAAAGAATCGAAGCAACGTCCGCAAACACGGCGTCGACTTCAATGATGCTATCGAAAT
CTTCAAGCATCCAGTGCTGACCGCTGCGGATCGCCGCGAGGATTTCGACGAAGATCGCTGGATTGCACTGGGGTGGATGA
AACTGATCGTGGCGCTGGCCGTATATGTAGAGCGCGACGGCGACATCGTACGGATTATTTCAGCACGCAAGGCAACGCGA
CCCGAGATAAAACGCTATGGAGACGCCATTTCTCAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9964.32 Da
• Isoelectric Point: 7.0127

>AT61361 WP_061998461.1 NZ_CP014505:c286532-286275 [Burkholderia sp. PAMC 28687]
MSKTTKTDWKRLETMPDSSIDTSEMPELGDEFFLNAELQRPPKQAVTMRLDADVLAWFKEQGEGYQTRINKLLRAYMQAQ
QKRHS

Nucleotide

• Length: 258 bp

>AT61361 NZ_CP014505:c286532-286275 [Burkholderia sp. PAMC 28687]
ATGAGCAAAACCACGAAGACCGACTGGAAGCGCCTGGAAACCATGCCGGATTCCAGCATCGATACAAGCGAGATGCCCGA
ACTGGGCGACGAGTTCTTCCTGAACGCCGAGTTGCAGAGACCGCCCAAGCAGGCGGTGACCATGCGTCTCGACGCCGACG
TGCTGGCCTGGTTCAAGGAACAAGGCGAGGGTTACCAGACACGCATCAACAAGCTTTTGCGCGCCTACATGCAGGCGCAA
CAAAAGCGCCACTCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A242M622