Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 73511..74112 Replicon plasmid pZH193
Accession NZ_CP014498
Organism Escherichia coli strain ZH193

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag AVR69_RS25670 Protein ID WP_001216034.1
Coordinates 73732..74112 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag AVR69_RS25665 Protein ID WP_001190712.1
Coordinates 73511..73732 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR69_RS25655 70492..71775 - 1284 WP_001617890.1 restriction endonuclease subunit S -
AVR69_RS25660 71772..73328 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
AVR69_RS25665 73511..73732 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AVR69_RS25670 73732..74112 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
AVR69_RS25675 74117..74296 + 180 WP_001513661.1 hypothetical protein -
AVR69_RS25680 74324..74683 + 360 WP_001513660.1 hypothetical protein -
AVR69_RS28315 74607..75020 + 414 Protein_86 DDE-type integrase/transposase/recombinase -
AVR69_RS25685 74970..75287 - 318 WP_001513659.1 hypothetical protein -
AVR69_RS25690 75515..76495 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
AVR69_RS25695 76739..78142 + 1404 WP_001373486.1 S-methylmethionine permease -
AVR69_RS25700 78129..79061 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / dfrA17 / aac(3)-IId / blaTEM-1B senB 1..209768 209768


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T61360 WP_001216034.1 NZ_CP014498:73732-74112 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T61360 NZ_CP014498:73732-74112 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT61360 WP_001190712.1 NZ_CP014498:73511-73732 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT61360 NZ_CP014498:73511-73732 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References