Detailed information of TA system TA061350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4992505..4993081
• Replicon: chromosome
• Accession: NZ_CP014497
• Organism: Escherichia coli strain ZH193

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A061KXE4
• Locus tag: AVR69_RS24945
• Protein ID: WP_001295743.1
• Coordinates: 4992505..4992792 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A066STF6
• Locus tag: AVR69_RS24950
• Protein ID: WP_000063148.1
• Coordinates: 4992779..4993081 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR69_RS24930	4988165..4989469	-	1305	WP_000535012.1	restriction endonuclease subunit S	-
AVR69_RS24935	4989459..4991078	-	1620	WP_001029745.1	type I restriction-modification system subunit M	-
AVR69_RS24940	4991142..4992308	-	1167	WP_000800831.1	restriction endonuclease	-
AVR69_RS24945	4992505..4992792	+	288	WP_001295743.1	BrnT family toxin	Toxin
AVR69_RS24950	4992779..4993081	+	303	WP_000063148.1	BrnA antitoxin family protein	Antitoxin
AVR69_RS24955	4993115..4994071	-	957	WP_001295745.1	GTPase	-
AVR69_RS24960	4994082..4994285	-	204	WP_000467859.1	YbdD/YjiX family protein	-
AVR69_RS24965	4994335..4996485	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11156.62 Da
• Isoelectric Point: 7.4697

>T61350 WP_001295743.1 NZ_CP014497:4992505-4992792 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T61350 NZ_CP014497:4992505-4992792 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11592.12 Da
• Isoelectric Point: 9.4646

>AT61350 WP_000063148.1 NZ_CP014497:4992779-4993081 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT61350 NZ_CP014497:4992779-4993081 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A061KXE4

Antitoxin

• AlphaFold DB: A0A066STF6