Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 55937..56457 Replicon chromosome
Accession NZ_CP014497
Organism Escherichia coli strain ZH193

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag AVR69_RS00275 Protein ID WP_001160964.1
Coordinates 56173..56457 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AVR69_RS00270 Protein ID WP_000125566.1
Coordinates 55937..56170 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR69_RS00245 51010..51297 + 288 WP_000203744.1 ferredoxin-like protein FixX -
AVR69_RS00250 51356..52687 + 1332 WP_001183209.1 MFS transporter -
AVR69_RS00255 52795..53325 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AVR69_RS00260 53318..55180 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
AVR69_RS00265 55372..55851 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AVR69_RS00270 55937..56170 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AVR69_RS00275 56173..56457 + 285 WP_001160964.1 CcdB family protein Toxin
AVR69_RS00280 56484..57332 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AVR69_RS00285 57339..57716 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AVR69_RS00290 57719..58540 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AVR69_RS00295 58537..59526 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AVR69_RS00300 59526..60812 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T61321 WP_001160964.1 NZ_CP014497:56173-56457 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T61321 NZ_CP014497:56173-56457 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT61321 WP_000125566.1 NZ_CP014497:55937-56170 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT61321 NZ_CP014497:55937-56170 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References