Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2241107..2241938 Replicon chromosome
Accession NZ_CP014495
Organism Escherichia coli strain SaT040

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag AVR72_RS11575 Protein ID WP_000854815.1
Coordinates 2241564..2241938 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag AVR72_RS11570 Protein ID WP_001280918.1
Coordinates 2241107..2241475 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR72_RS11530 2236698..2237375 + 678 WP_001362823.1 hypothetical protein -
AVR72_RS11535 2237391..2237801 + 411 WP_000846703.1 hypothetical protein -
AVR72_RS11540 2238022..2238840 + 819 WP_001542275.1 DUF945 domain-containing protein -
AVR72_RS11545 2238840..2239085 + 246 WP_001164966.1 hypothetical protein -
AVR72_RS11550 2239179..2239652 + 474 WP_001542276.1 antirestriction protein -
AVR72_RS11555 2239668..2240144 + 477 WP_001186200.1 RadC family protein -
AVR72_RS11560 2240207..2240428 + 222 WP_000692345.1 DUF987 domain-containing protein -
AVR72_RS11565 2240447..2241091 + 645 WP_000086752.1 hypothetical protein -
AVR72_RS11570 2241107..2241475 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AVR72_RS11575 2241564..2241938 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
AVR72_RS11580 2241935..2242129 + 195 WP_000988600.1 hypothetical protein -
AVR72_RS28715 2242142..2242255 + 114 WP_001161660.1 DUF957 domain-containing protein -
AVR72_RS11590 2242544..2242690 - 147 Protein_2222 transposase domain-containing protein -
AVR72_RS11595 2242744..2242926 + 183 WP_001296208.1 hypothetical protein -
AVR72_RS11600 2243027..2243356 - 330 WP_000450409.1 DUF496 family protein -
AVR72_RS11605 2243528..2244586 - 1059 WP_001200889.1 FUSC family protein -
AVR72_RS11610 2244784..2245257 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
AVR72_RS11615 2245376..2246542 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T61309 WP_000854815.1 NZ_CP014495:2241564-2241938 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T61309 NZ_CP014495:2241564-2241938 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT61309 WP_001280918.1 NZ_CP014495:2241107-2241475 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT61309 NZ_CP014495:2241107-2241475 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References