Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 124726..125273 Replicon plasmid pG749_1
Accession NZ_CP014489
Organism Escherichia coli strain G749

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag AVR74_RS24975 Protein ID WP_000969996.1
Coordinates 124726..125007 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag AVR74_RS24980 Protein ID WP_000079941.1
Coordinates 125004..125273 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR74_RS24945 120157..120417 + 261 WP_000343091.1 hypothetical protein -
AVR74_RS24950 120414..121004 + 591 WP_001518980.1 hypothetical protein -
AVR74_RS24955 121024..121281 - 258 WP_000142431.1 hypothetical protein -
AVR74_RS24960 121410..121745 + 336 WP_001080729.1 colicin 1A immunity protein -
AVR74_RS24965 121767..123647 - 1881 WP_001283335.1 colicin -
AVR74_RS24970 123833..124680 - 848 Protein_127 3'-5' exonuclease -
AVR74_RS24975 124726..125007 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AVR74_RS24980 125004..125273 - 270 WP_000079941.1 hypothetical protein Antitoxin
AVR74_RS24985 125917..128883 - 2967 WP_001138014.1 Tn3-like element TnAs1 family transposase -
AVR74_RS24990 128887..129447 - 561 WP_001161490.1 recombinase family protein -
AVR74_RS29135 129623..129973 - 351 WP_000454193.1 DUF3330 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / dfrA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN 1..149732 149732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T61292 WP_000969996.1 NZ_CP014489:c125007-124726 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T61292 NZ_CP014489:c125007-124726 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT61292 WP_000079941.1 NZ_CP014489:c125273-125004 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT61292 NZ_CP014489:c125273-125004 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References