Detailed information of TA system TA061284

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3240524..3241359
• Replicon: chromosome
• Accession: NZ_CP014488
• Organism: Escherichia coli strain G749

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7A2ZCB4
• Locus tag: AVR74_RS16185
• Protein ID: WP_061157978.1
• Coordinates: 3240982..3241359 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: AVR74_RS16180
• Protein ID: WP_001285610.1
• Coordinates: 3240524..3240892 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR74_RS16160	3238249..3239067	+	819	WP_061157976.1	DUF945 domain-containing protein	-
AVR74_RS16165	3239159..3239644	+	486	WP_061157977.1	antirestriction protein	-
AVR74_RS16170	3239660..3240136	+	477	WP_001186774.1	RadC family protein	-
AVR74_RS16175	3240223..3240444	+	222	WP_000692350.1	DUF987 domain-containing protein	-
AVR74_RS16180	3240524..3240892	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AVR74_RS16185	3240982..3241359	+	378	WP_061157978.1	type IV toxin-antitoxin system toxin CbtA	Toxin
AVR74_RS28745	3241356..3241553	+	198	Protein_3113	hypothetical protein	-
AVR74_RS28750	3241581..3241778	+	198	WP_085949090.1	DUF957 domain-containing protein	-
AVR74_RS16200	3241863..3242705	+	843	WP_001280493.1	DUF4942 domain-containing protein	-
AVR74_RS16205	3243047..3243217	+	171	Protein_3116	IS110 family transposase	-
AVR74_RS16210	3244028..3245011	+	984	WP_061157979.1	KpsF/GutQ family sugar-phosphate isomerase	-
AVR74_RS16215	3245083..3246231	+	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13911.90 Da
• Isoelectric Point: 7.9085

>T61284 WP_061157978.1 NZ_CP014488:3240982-3241359 [Escherichia coli]
MKTLPVLPGQAASSHPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T61284 NZ_CP014488:3240982-3241359 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACACCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT61284 WP_001285610.1 NZ_CP014488:3240524-3240892 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT61284 NZ_CP014488:3240524-3240892 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7A2ZCB4