Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2079747..2080578 Replicon chromosome
Accession NZ_CP014488
Organism Escherichia coli strain G749

Toxin (Protein)


Gene name yeeV Uniprot ID A0A768PNB2
Locus tag AVR74_RS10510 Protein ID WP_061157912.1
Coordinates 2080204..2080578 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A785EPM7
Locus tag AVR74_RS10505 Protein ID WP_001535568.1
Coordinates 2079747..2080115 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR74_RS10465 2074804..2075874 + 1071 WP_061157908.1 patatin-like phospholipase family protein -
AVR74_RS10470 2076010..2076687 + 678 WP_001362823.1 hypothetical protein -
AVR74_RS10475 2076703..2077113 + 411 WP_061157909.1 hypothetical protein -
AVR74_RS10480 2077334..2078155 + 822 WP_001234571.1 DUF945 domain-containing protein -
AVR74_RS10490 2078418..2078897 + 480 WP_061157910.1 antirestriction protein -
AVR74_RS10495 2078913..2079389 + 477 WP_001186786.1 RadC family protein -
AVR74_RS10500 2079452..2079673 + 222 WP_061157911.1 DUF987 domain-containing protein -
AVR74_RS10505 2079747..2080115 + 369 WP_001535568.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
AVR74_RS10510 2080204..2080578 + 375 WP_061157912.1 type IV toxin-antitoxin system toxin CbtA Toxin
AVR74_RS10515 2080575..2080769 + 195 WP_000988601.1 hypothetical protein -
AVR74_RS28640 2080782..2080895 + 114 WP_001161660.1 DUF957 domain-containing protein -
AVR74_RS10525 2081184..2081330 - 147 Protein_2011 transposase domain-containing protein -
AVR74_RS10530 2081384..2081566 + 183 WP_061157913.1 ethanolamine utilization protein -
AVR74_RS10535 2081667..2081996 - 330 WP_000450409.1 DUF496 family protein -
AVR74_RS10540 2082168..2083226 - 1059 WP_001200890.1 FUSC family protein -
AVR74_RS10545 2083424..2083897 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
AVR74_RS10550 2084016..2085182 - 1167 WP_061157914.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13871.91 Da        Isoelectric Point: 7.8522

>T61281 WP_061157912.1 NZ_CP014488:2080204-2080578 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNSITLGKYPEAK

Download         Length: 375 bp

>T61281 NZ_CP014488:2080204-2080578 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAGCATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13481.26 Da        Isoelectric Point: 5.0468

>AT61281 WP_001535568.1 NZ_CP014488:2079747-2080115 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLSSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLACEADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT61281 NZ_CP014488:2079747-2080115 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
CCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCAATCAGCATACCGTCACGCTGTATGCGAAGGGGCTGGCCTGTGAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A768PNB2

Antitoxin

Source ID Structure
AlphaFold DB A0A785EPM7

References