Detailed information of TA system TA061263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2344708..2345686
• Replicon: chromosome
• Accession: NZ_CP014486
• Organism: Bacillus cereus strain FORC021

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: FORC21_RS12540
• Protein ID: WP_000624975.1
• Coordinates: 2344708..2345445 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: FORC21_RS12545
• Protein ID: WP_000237818.1
• Coordinates: 2345558..2345686 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC21_RS12515	2340350..2341132	+	783	Protein_2297	class I SAM-dependent methyltransferase	-
FORC21_RS12520	2341290..2342966	-	1677	WP_139256968.1	alpha-keto acid decarboxylase family protein	-
FORC21_RS12525	2343083..2343565	+	483	WP_072770281.1	MarR family winged helix-turn-helix transcriptional regulator	-
FORC21_RS12530	2343732..2344469	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FORC21_RS12540	2344708..2345445	+	738	WP_000624975.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FORC21_RS12545	2345558..2345686	+	129	WP_000237818.1	hypothetical protein	Antitoxin
FORC21_RS12550	2345759..2345935	+	177	WP_065382523.1	stage II sporulation protein SB	-
FORC21_RS12555	2345955..2346344	-	390	WP_000713862.1	YxeA family protein	-
FORC21_RS12560	2346556..2348025	+	1470	WP_065382524.1	beta-Ala-His dipeptidase	-
FORC21_RS12565	2348271..2349080	+	810	WP_065382626.1	C39 family peptidase	-
FORC21_RS12570	2349106..2349708	+	603	WP_065382625.1	hypothetical protein	-
FORC21_RS12575	2349951..2350202	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28278.98 Da
• Isoelectric Point: 8.2998

>T61263 WP_000624975.1 NZ_CP014486:2344708-2345445 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMAIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T61263 NZ_CP014486:2344708-2345445 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACTGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGCTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCTGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT61263 WP_000237818.1 NZ_CP014486:2345558-2345686 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT61263 NZ_CP014486:2345558-2345686 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5