Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240211..240853 Replicon chromosome
Accession NZ_CP014486
Organism Bacillus cereus strain FORC021

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FORC21_RS01415 Protein ID WP_000635963.1
Coordinates 240503..240853 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FORC21_RS01410 Protein ID WP_000004570.1
Coordinates 240211..240498 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC21_RS01385 235528..236490 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
FORC21_RS01390 236483..237055 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
FORC21_RS01395 237149..237508 + 360 WP_000583416.1 holo-ACP synthase -
FORC21_RS01400 237665..238615 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
FORC21_RS01405 238733..239902 + 1170 WP_000390615.1 alanine racemase -
FORC21_RS01410 240211..240498 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FORC21_RS01415 240503..240853 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FORC21_RS01420 240921..243089 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
FORC21_RS01425 243147..243263 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FORC21_RS01430 243459..243917 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T61262 WP_000635963.1 NZ_CP014486:240503-240853 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T61262 NZ_CP014486:240503-240853 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT61262 WP_000004570.1 NZ_CP014486:240211-240498 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT61262 NZ_CP014486:240211-240498 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References