Detailed information of TA system TA061260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 33204..33792
• Replicon: plasmid pOXA58-AP_882
• Accession: NZ_CP014479
• Organism: Acinetobacter pittii strain AP_882

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: AYJ52_RS19725
• Protein ID: WP_000438825.1
• Coordinates: 33204..33491 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: AYJ52_RS19730
• Protein ID: WP_004728120.1
• Coordinates: 33478..33792 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ52_RS19695	28570..29412	+	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
AYJ52_RS19700	29466..30494	+	1029	WP_081106519.1	transposase	-
AYJ52_RS20545	30609..31361	+	753	Protein_32	IS1-like element ISAba3 family transposase	-
AYJ52_RS19715	31489..32316	+	828	WP_000073658.1	AraC family transcriptional regulator	-
AYJ52_RS19720	32366..32971	+	606	WP_000447788.1	LysE family translocator	-
AYJ52_RS19725	33204..33491	+	288	WP_000438825.1	BrnT family toxin	Toxin
AYJ52_RS19730	33478..33792	+	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
AYJ52_RS19735	33881..34411	-	531	WP_000172759.1	hypothetical protein	-
AYJ52_RS20550	34560..34862	+	303	Protein_38	type II toxin-antitoxin system RelE/ParE family toxin	-
AYJ52_RS19740	34863..35144	+	282	WP_000985609.1	putative addiction module antidote protein	-
AYJ52_RS19745	35224..35643	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
AYJ52_RS19750	35785..36105	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AYJ52_RS19755	36098..36370	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / blaOXA-58	-	1..36862	36862
	-	inside	IScluster/Tn	blaOXA-58	-	22342..31046	8704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T61260 WP_000438825.1 NZ_CP014479:33204-33491 [Acinetobacter pittii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T61260 NZ_CP014479:33204-33491 [Acinetobacter pittii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT61260 WP_004728120.1 NZ_CP014479:33478-33792 [Acinetobacter pittii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT61260 NZ_CP014479:33478-33792 [Acinetobacter pittii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9