Detailed information of TA system TA061259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 25239..25827
• Replicon: plasmid pOXA58-AP_882
• Accession: NZ_CP014479
• Organism: Acinetobacter pittii strain AP_882

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: AYJ52_RS19670
• Protein ID: WP_004647749.1
• Coordinates: 25239..25535 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AYJ52_RS19675
• Protein ID: WP_004969304.1
• Coordinates: 25537..25827 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ52_RS19630	20355..20627	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
AYJ52_RS19635	20620..20940	-	321	WP_004698338.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AYJ52_RS19640	21107..21367	+	261	WP_004698336.1	hypothetical protein	-
AYJ52_RS19645	21643..21867	-	225	WP_004676219.1	helix-turn-helix domain-containing protein	-
AYJ52_RS19650	22018..22263	+	246	WP_004282220.1	hypothetical protein	-
AYJ52_RS19655	22342..23046	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
AYJ52_RS19660	23109..23501	+	393	Protein_23	recombinase family protein	-
AYJ52_RS19665	23722..24795	+	1074	WP_043041639.1	hypothetical protein	-
AYJ52_RS19670	25239..25535	+	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYJ52_RS19675	25537..25827	+	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
AYJ52_RS19680	25938..27392	-	1455	WP_026438532.1	SulP family inorganic anion transporter	-
AYJ52_RS19685	27642..27851	-	210	WP_004976362.1	hypothetical protein	-
AYJ52_RS19690	28121..28489	-	369	Protein_29	IS1-like element ISAba3 family transposase	-
AYJ52_RS19695	28570..29412	+	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
AYJ52_RS19700	29466..30494	+	1029	WP_081106519.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / blaOXA-58	-	1..36862	36862
	-	inside	IScluster/Tn	blaOXA-58	-	22342..31046	8704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T61259 WP_004647749.1 NZ_CP014479:25239-25535 [Acinetobacter pittii]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T61259 NZ_CP014479:25239-25535 [Acinetobacter pittii]
ATGATTGAAATTAAACGTCTTCCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATCCGCCTGAA
TCGTCGATTAGATAAGGTTCAACGTGGAAACTGGGGAGACATTAAACCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTTCTGGATGGAGGATGTATTACATCCAACATGGTGACGTTGTGATTGTAATGCTTGGTGGTGGAGATAAATCA
ACTCAACAACAAGACATCGATCGGGCAGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT61259 WP_004969304.1 NZ_CP014479:25537-25827 [Acinetobacter pittii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT61259 NZ_CP014479:25537-25827 [Acinetobacter pittii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCGCTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGAAAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TCGCTAAAGAAGCAGGGATTGGGCGTGAAGCACTCTACAAAGCCCTACGTCAGGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAGCTCACAGTACAACACGCTTAA