Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 4533045..4533625 Replicon chromosome
Accession NZ_CP014476
Organism Methylomonas denitrificans strain FJG1

Toxin (Protein)


Gene name PumA Uniprot ID A0A140E683
Locus tag JT25_RS20875 Protein ID WP_036279563.1
Coordinates 4533326..4533625 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A140E682
Locus tag JT25_RS20870 Protein ID WP_036279565.1
Coordinates 4533045..4533323 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT25_RS20840 4528564..4528962 + 399 WP_036279579.1 hypothetical protein -
JT25_RS20845 4529009..4529557 - 549 WP_064044669.1 hypothetical protein -
JT25_RS20850 4529580..4530782 - 1203 WP_036279573.1 hypothetical protein -
JT25_RS20855 4530793..4531149 - 357 WP_036279568.1 hypothetical protein -
JT25_RS20860 4531477..4531947 - 471 WP_132325300.1 hypothetical protein -
JT25_RS20865 4532519..4532716 - 198 Protein_4152 hypothetical protein -
JT25_RS20870 4533045..4533323 - 279 WP_036279565.1 putative addiction module antidote protein Antitoxin
JT25_RS20875 4533326..4533625 - 300 WP_036279563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JT25_RS20880 4533671..4534984 - 1314 WP_036279560.1 integrase family protein -
JT25_RS20890 4535568..4536727 + 1160 Protein_4156 IS3 family transposase -
JT25_RS20895 4536802..4537677 + 876 WP_036280151.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4533045..4539957 6912


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(45-89)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11502.20 Da        Isoelectric Point: 9.8183

>T61255 WP_036279563.1 NZ_CP014476:c4533625-4533326 [Methylomonas denitrificans]
MKYELQSTQTFNRWLSGLKDRTVKNQLLSRLARMENGHFGDYKPLSADLFELRCFFGAGLRIYYTVRNQQVILLLVGGDK
STQDRDIEKAQTILKSLEN

Download         Length: 300 bp

>T61255 NZ_CP014476:c4533625-4533326 [Methylomonas denitrificans]
ATGAAATACGAATTGCAGAGTACCCAAACTTTTAACCGATGGCTATCTGGATTGAAAGACCGGACAGTCAAAAACCAATT
GCTGTCACGGCTAGCGCGAATGGAAAACGGCCATTTTGGAGACTACAAGCCTTTATCCGCCGATTTGTTTGAATTGCGCT
GCTTCTTTGGTGCGGGCCTGCGGATTTATTACACGGTACGCAATCAGCAGGTAATTCTATTGCTGGTTGGCGGCGACAAA
TCCACCCAAGACAGGGATATTGAAAAAGCCCAAACCATTTTGAAAAGTCTGGAGAATTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 9829.06 Da        Isoelectric Point: 4.5836

>AT61255 WP_036279565.1 NZ_CP014476:c4533323-4533045 [Methylomonas denitrificans]
MSETITSFDIAEHLETDADIQAFLRETAANGDVSDFIHALNTAARAKGMTEVAKQAGVTRASLYKSLADDGNPRFETIAK
IVEALGCKLVVS

Download         Length: 279 bp

>AT61255 NZ_CP014476:c4533323-4533045 [Methylomonas denitrificans]
ATGAGTGAAACCATCACCTCGTTCGACATTGCCGAACATCTGGAAACCGACGCTGATATTCAGGCCTTTTTGCGTGAAAC
AGCAGCCAACGGCGATGTGTCCGATTTTATCCACGCATTAAACACCGCAGCCCGCGCCAAAGGTATGACCGAAGTTGCCA
AGCAAGCCGGCGTAACAAGAGCCAGTCTTTATAAATCCTTGGCTGATGATGGCAACCCCCGCTTTGAAACCATTGCAAAA
ATTGTCGAGGCATTGGGTTGCAAGTTGGTGGTGTCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140E683

Antitoxin

Source ID Structure
AlphaFold DB A0A140E682

References