Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/-
Location 4134039..4134628 Replicon chromosome
Accession NZ_CP014476
Organism Methylomonas denitrificans strain FJG1

Toxin (Protein)


Gene name vapC Uniprot ID A0A126T8W7
Locus tag JT25_RS18930 Protein ID WP_036276842.1
Coordinates 4134245..4134628 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A126T938
Locus tag JT25_RS18925 Protein ID WP_036276844.1
Coordinates 4134039..4134248 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT25_RS18905 4129131..4129940 + 810 WP_036275486.1 ABC transporter permease -
JT25_RS18910 4130152..4130973 + 822 WP_036275485.1 hypothetical protein -
JT25_RS18915 4131327..4131848 + 522 WP_036275484.1 DUF1097 domain-containing protein -
JT25_RS18920 4132294..4133841 + 1548 WP_062329407.1 2-isopropylmalate synthase -
JT25_RS18925 4134039..4134248 + 210 WP_036276844.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JT25_RS18930 4134245..4134628 + 384 WP_036276842.1 PIN domain-containing protein Toxin
JT25_RS18935 4134940..4136292 - 1353 WP_036276838.1 hypothetical protein -
JT25_RS18940 4136444..4136701 - 258 WP_148655021.1 hypothetical protein -
JT25_RS18950 4136978..4138207 - 1230 WP_062329409.1 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4136978..4138207 1229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14342.71 Da        Isoelectric Point: 5.1468

>T61254 WP_036276842.1 NZ_CP014476:4134245-4134628 [Methylomonas denitrificans]
MILVDTSVWVNHFKDRNEDLVRLLLSDSVFIHPLIIAELACGTPPAPRTQTLNNLRQLKVCNQASLQEMEEFIEREALFG
LDCELIDLQVLASVLITPGAKLWTLNKRLAKLAERFGVAYPTWPTAN

Download         Length: 384 bp

>T61254 NZ_CP014476:4134245-4134628 [Methylomonas denitrificans]
ATGATATTGGTCGATACCTCGGTTTGGGTTAACCATTTCAAAGATCGTAACGAAGACTTGGTTCGGTTACTACTTTCCGA
TTCGGTGTTTATTCACCCGCTGATCATCGCAGAATTGGCCTGCGGTACACCGCCCGCGCCGAGAACCCAAACTCTGAACA
ACCTGCGCCAGTTAAAAGTCTGCAATCAAGCCAGTTTGCAGGAAATGGAAGAATTTATCGAGCGCGAAGCACTGTTTGGC
TTGGATTGCGAGCTAATAGATTTACAGGTACTGGCATCGGTATTGATCACACCCGGCGCAAAGCTGTGGACGCTGAACAA
GCGCTTAGCGAAACTAGCCGAGCGCTTTGGTGTGGCTTATCCAACATGGCCGACCGCGAATTGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 7775.94 Da        Isoelectric Point: 4.7085

>AT61254 WP_036276844.1 NZ_CP014476:4134039-4134248 [Methylomonas denitrificans]
MRTTVTIDDTLYQKALEMADPDMDKAEIFREAMKTFVRIQAAKRLAALGGTMPDMADIPRERGQTVQSE

Download         Length: 210 bp

>AT61254 NZ_CP014476:4134039-4134248 [Methylomonas denitrificans]
ATGCGAACCACCGTAACCATTGATGACACCTTGTACCAAAAAGCCTTGGAAATGGCCGACCCGGATATGGACAAGGCCGA
AATCTTCCGGGAAGCGATGAAAACTTTCGTGCGGATTCAGGCCGCTAAACGCTTGGCCGCCTTGGGCGGAACCATGCCGG
ATATGGCCGATATTCCCCGAGAACGCGGCCAAACCGTCCAGTCCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A126T8W7

Antitoxin

Source ID Structure
AlphaFold DB A0A126T938

References