Detailed information of TA system TA061252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2649273..2650060
• Replicon: chromosome
• Accession: NZ_CP014476
• Organism: Methylomonas denitrificans strain FJG1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A126T562
• Locus tag: JT25_RS12145
• Protein ID: WP_036280388.1
• Coordinates: 2649273..2649773 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JT25_RS24005
• Protein ID: WP_082769223.1
• Coordinates: 2649770..2650060 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT25_RS12130	2646951..2647853	+	903	WP_036278027.1	MBL fold metallo-hydrolase	-
JT25_RS12135	2647992..2649118	-	1127	Protein_2440	IS3 family transposase	-
JT25_RS12145	2649273..2649773	-	501	WP_036280388.1	GNAT family N-acetyltransferase	Toxin
JT25_RS24005	2649770..2650060	-	291	WP_082769223.1	DUF1778 domain-containing protein	Antitoxin
JT25_RS25075	2650248..2650574	+	327	WP_157200633.1	hypothetical protein	-
JT25_RS24015	2650804..2651028	+	225	Protein_2444	recombinase family protein	-
JT25_RS12155	2651090..2652604	+	1515	WP_062329834.1	IS21 family transposase	-
JT25_RS12160	2652594..2653343	+	750	WP_062328302.1	IS21-like element helper ATPase IstB	-
JT25_RS12165	2653392..2653655	-	264	WP_062328738.1	hypothetical protein	-
JT25_RS12175	2653732..2654817	+	1086	Protein_2448	IS3 family transposase	-
JT25_RS24745	2654860..2654997	-	138	Protein_2449	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2648813..2657025	8212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-89)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18655.52 Da
• Isoelectric Point: 10.2717

>T61252 WP_036280388.1 NZ_CP014476:c2649773-2649273 [Methylomonas denitrificans]
MNHCRPESLGALHHLEFFDCGKPALNEWLIRHARQTQGSGSAKTFVTTDDTQRVIGYFSLTVGQIDTLDAPERIRKGMGQ
YPIPVAILARLAVSKNHQGRGIGIGLLQDAIRRSLLIAEQAGIRAMLTHPIDDEAARFYRRFGFIASPLQEQQLLLLLKD
ARRWAR

Nucleotide

• Length: 501 bp

>T61252 NZ_CP014476:c2649773-2649273 [Methylomonas denitrificans]
TTGAATCATTGTCGCCCTGAGTCCCTTGGGGCATTGCACCATCTAGAATTCTTTGACTGTGGCAAACCAGCACTTAACGA
ATGGTTGATTCGTCATGCCAGGCAGACACAGGGCAGTGGCTCAGCTAAAACCTTCGTTACTACGGACGATACTCAACGAG
TCATTGGCTATTTCAGTTTGACTGTTGGGCAGATCGACACGTTGGATGCCCCTGAGCGCATTCGCAAAGGCATGGGACAA
TACCCCATTCCAGTTGCGATTCTTGCTCGTTTGGCGGTTTCCAAAAATCACCAAGGGCGCGGTATTGGTATCGGATTATT
GCAGGATGCTATTCGAAGATCGCTGCTTATTGCTGAGCAAGCAGGTATCCGGGCAATGTTAACTCACCCGATTGATGATG
AGGCCGCTCGTTTTTACAGACGTTTTGGATTTATTGCCTCTCCCTTACAAGAACAACAGCTTCTGTTACTGCTTAAAGAC
GCGAGGCGCTGGGCCAGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10555.81 Da
• Isoelectric Point: 4.4217

>AT61252 WP_082769223.1 NZ_CP014476:c2650060-2649770 [Methylomonas denitrificans]
MTITATVATRSARLGLRATPEQEAVLRRAAEVAHKSLTDFILDSACQAAEQTLLDQRLFMVSGGQYQAFLDLLDGPSEEN
EGLRDLFSRTAPWDNS

Nucleotide

• Length: 291 bp

>AT61252 NZ_CP014476:c2650060-2649770 [Methylomonas denitrificans]
ATGACAATTACAGCCACTGTTGCAACCCGATCTGCGCGATTGGGTCTGCGAGCTACACCAGAGCAAGAAGCGGTATTACG
TCGAGCCGCCGAGGTGGCACATAAGTCATTAACCGACTTTATTCTTGATAGCGCTTGCCAAGCAGCAGAACAAACCTTGC
TCGACCAACGATTGTTTATGGTGTCAGGCGGCCAATATCAGGCTTTTTTGGATTTATTGGATGGTCCAAGTGAAGAAAAT
GAGGGATTACGTGACTTGTTTTCCCGAACTGCTCCCTGGGACAATTCTTGA

Structures

Toxin

• AlphaFold DB: A0A126T562