Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4823621..4824396 Replicon chromosome
Accession NZ_CP014475
Organism Mycolicibacterium phlei strain CCUG 21000

Toxin (Protein)


Gene name tacT Uniprot ID A0A5N5VD23
Locus tag MPHLCCUG_RS23165 Protein ID WP_003887343.1
Coordinates 4823896..4824396 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5N5VFQ7
Locus tag MPHLCCUG_RS23160 Protein ID WP_003887344.1
Coordinates 4823621..4823899 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPHLCCUG_RS23140 4819978..4821618 - 1641 WP_061492323.1 hypothetical protein -
MPHLCCUG_RS23145 4821811..4822380 - 570 WP_003887347.1 dCTP deaminase -
MPHLCCUG_RS23150 4822463..4822789 - 327 WP_003887345.1 hypothetical protein -
MPHLCCUG_RS23160 4823621..4823899 + 279 WP_003887344.1 DUF1778 domain-containing protein Antitoxin
MPHLCCUG_RS23165 4823896..4824396 + 501 WP_003887343.1 GNAT family N-acetyltransferase Toxin
MPHLCCUG_RS23170 4824528..4824851 + 324 WP_061482428.1 hypothetical protein -
MPHLCCUG_RS23175 4824922..4825584 - 663 WP_061482411.1 hypothetical protein -
MPHLCCUG_RS23180 4826094..4828346 + 2253 WP_061482412.1 PE-PPE domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(37-142)

Antitoxin

Vibrio phage ICP1, Orf50

(8-84)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18725.35 Da        Isoelectric Point: 9.0973

>T61247 WP_003887343.1 NZ_CP014475:4823896-4824396 [Mycolicibacterium phlei]
VSYSQPRPIREDDEVGHFDCGDDPLNTYLIKKAVSNHRSGASRCFVTCREGRVVGYYALAAGSVERKAVSGRFRRNMPDP
IPVILLTRLAVDRREQGKGLGRHLLRDAITRTVYVADHIGARAMLVHAIDEDARNFYSRFDFEQSPTDPLHLLLSIKDAR
ILITPH

Download         Length: 501 bp

>T61247 NZ_CP014475:4823896-4824396 [Mycolicibacterium phlei]
GTGAGCTACAGCCAGCCCCGGCCAATCCGCGAGGACGACGAGGTAGGCCACTTCGACTGCGGAGACGACCCTCTTAACAC
CTACCTGATCAAGAAGGCTGTCTCCAATCACCGAAGCGGTGCCTCACGTTGCTTCGTCACGTGCCGCGAGGGCCGGGTCG
TGGGGTACTACGCACTGGCGGCGGGCTCCGTGGAACGCAAGGCGGTGTCGGGCAGGTTTCGGCGGAATATGCCCGATCCG
ATACCCGTGATTCTGCTAACCCGTCTGGCGGTCGACCGCAGGGAGCAGGGCAAGGGTCTGGGCAGACATCTCCTGCGTGA
TGCGATTACGCGGACTGTCTACGTTGCCGACCATATTGGCGCACGGGCGATGCTTGTACACGCCATCGATGAAGACGCCC
GCAACTTCTATTCGCGTTTCGACTTCGAGCAATCACCGACCGATCCGCTGCACCTGCTGCTGTCCATCAAGGATGCCCGA
ATCTTGATCACTCCGCACTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10358.72 Da        Isoelectric Point: 5.1473

>AT61247 WP_003887344.1 NZ_CP014475:4823621-4823899 [Mycolicibacterium phlei]
MAATKTERFAVRLTSEQDALIRRAAEVKGIDLTSFTLSATLAHARDVLADRTYFALGTSAWEEFLEVLDRPVTHKPQLEK
LFSERSIFDSEA

Download         Length: 279 bp

>AT61247 NZ_CP014475:4823621-4823899 [Mycolicibacterium phlei]
ATGGCTGCAACCAAGACGGAGCGTTTCGCCGTGCGCCTGACTTCTGAGCAGGACGCGCTGATTCGTCGTGCAGCGGAGGT
CAAAGGAATTGATCTCACCAGCTTCACCCTGAGCGCGACGCTGGCCCATGCTCGTGACGTCCTTGCGGACCGCACGTACT
TCGCGTTGGGCACGTCCGCTTGGGAGGAGTTCCTGGAAGTTCTGGATCGGCCTGTCACGCATAAGCCGCAACTGGAGAAA
CTTTTCTCTGAACGGTCGATCTTCGACTCCGAGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5N5VD23

Antitoxin

Source ID Structure
AlphaFold DB A0A5N5VFQ7

References