Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pezAT/zeta-ParD
Location 2580257..2581245 Replicon chromosome
Accession NZ_CP014475
Organism Mycolicibacterium phlei strain CCUG 21000

Toxin (Protein)


Gene name pezT Uniprot ID A0A5N5US87
Locus tag MPHLCCUG_RS12330 Protein ID WP_003888374.1
Coordinates 2580257..2580835 (-) Length 193 a.a.

Antitoxin (Protein)


Gene name pezA Uniprot ID A0A5N5US60
Locus tag MPHLCCUG_RS12335 Protein ID WP_003888373.1
Coordinates 2580856..2581245 (-) Length 130 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPHLCCUG_RS12320 2575961..2578099 - 2139 WP_003888376.1 topoisomerase IV -
MPHLCCUG_RS12325 2578110..2580143 - 2034 WP_003888375.1 DNA gyrase subunit B -
MPHLCCUG_RS12330 2580257..2580835 - 579 WP_003888374.1 zeta toxin family protein Toxin
MPHLCCUG_RS12335 2580856..2581245 - 390 WP_003888373.1 hypothetical protein Antitoxin
MPHLCCUG_RS12340 2581390..2581779 + 390 Protein_2463 FAD-dependent oxidoreductase -
MPHLCCUG_RS12345 2581776..2582360 + 585 WP_061481325.1 TetR/AcrR family transcriptional regulator -
MPHLCCUG_RS12350 2582391..2583296 + 906 WP_110766383.1 aromatic alcohol reductase -
MPHLCCUG_RS26395 2583298..2583459 - 162 WP_003888369.1 hypothetical protein -
MPHLCCUG_RS12355 2583464..2584348 - 885 WP_050982670.1 SAM-dependent methyltransferase -
MPHLCCUG_RS12360 2584442..2585065 - 624 WP_003888367.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(5-134)

Antitoxin

ParD-like antitoxin of type II bacterial toxin-antitoxin system

(6-106)


Sequences

Toxin        


Download         Length: 193 a.a.        Molecular weight: 21195.41 Da        Isoelectric Point: 10.3743

>T61246 WP_003888374.1 NZ_CP014475:c2580835-2580257 [Mycolicibacterium phlei]
MRRLDLVVGPNGAGKSTFVGFTLAPLLPRSVFVNADEIAKLRWPDDPLGHAYDAARIAAETRAKLIETGVSFIAETVFSH
PSKLDLIHTARDAGYTVALHVVLIPENLAVERVKRRVRHGGHDVPEVKIRQRHRRLWPLVAEAIEIVDTATVYDNSQRKG
PRIVAQFTAGVVVGAPAWPVWTSEHLAGRWPA

Download         Length: 579 bp

>T61246 NZ_CP014475:c2580835-2580257 [Mycolicibacterium phlei]
ATGAGGCGGCTGGACCTGGTCGTCGGTCCCAACGGTGCCGGCAAGTCGACGTTCGTCGGATTCACCCTGGCGCCACTGCT
GCCGCGAAGCGTGTTCGTCAACGCCGACGAGATCGCCAAGCTGCGATGGCCCGACGACCCACTCGGGCACGCCTACGACG
CCGCCAGGATCGCCGCCGAGACCCGCGCCAAGCTGATCGAAACCGGCGTGTCGTTCATCGCCGAGACCGTCTTCTCCCAC
CCGTCCAAGCTCGACCTGATCCACACCGCCCGTGACGCCGGCTACACCGTCGCACTTCACGTGGTCCTGATCCCCGAGAA
TCTCGCGGTCGAGCGTGTCAAGCGGCGTGTTCGCCACGGCGGGCACGACGTTCCCGAGGTCAAGATCCGGCAACGACACC
GGCGGCTGTGGCCGCTGGTGGCCGAGGCGATCGAAATCGTCGACACGGCAACGGTATACGACAACTCCCAGCGCAAGGGC
CCGCGTATCGTCGCGCAGTTCACCGCGGGTGTCGTGGTCGGCGCGCCGGCGTGGCCGGTGTGGACGTCCGAGCATCTGGC
TGGTCGCTGGCCGGCCTGA

Antitoxin


Download         Length: 130 a.a.        Molecular weight: 13626.95 Da        Isoelectric Point: 4.5530

>AT61246 WP_003888373.1 NZ_CP014475:c2581245-2580856 [Mycolicibacterium phlei]
MGNTADRVTRVAADLMEAAAAEGARQSRSAKQQLDHWARVGRAVSSHQSAARHRVEAALAGRLDAGELSEEEGVVFNAEI
AAAIEESLLTADYGETLAAQGITTVALDDDGRIVEYRPDGTAAVVHTDR

Download         Length: 390 bp

>AT61246 NZ_CP014475:c2581245-2580856 [Mycolicibacterium phlei]
ATGGGCAACACCGCAGATCGCGTCACGCGGGTCGCCGCCGATCTGATGGAGGCCGCGGCCGCCGAGGGCGCTCGCCAGAG
CAGATCGGCGAAACAGCAACTCGATCACTGGGCACGGGTCGGTCGCGCGGTATCGAGCCACCAGTCCGCGGCCCGCCATC
GCGTCGAGGCCGCGCTGGCCGGCCGCCTGGACGCCGGCGAGCTGTCCGAGGAGGAGGGCGTCGTCTTCAACGCCGAGATC
GCCGCGGCCATCGAGGAATCCCTGCTCACCGCCGACTACGGGGAGACCCTCGCGGCGCAGGGCATCACGACGGTGGCCCT
CGACGACGACGGCAGGATCGTCGAGTACCGACCCGACGGCACCGCCGCCGTGGTGCACACCGACCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5N5US87

Antitoxin

Source ID Structure
AlphaFold DB A0A5N5US60

References