Detailed information of TA system TA061244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 814655..815301
• Replicon: chromosome
• Accession: NZ_CP014475
• Organism: Mycolicibacterium phlei strain CCUG 21000

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MPHLCCUG_RS04105
• Protein ID: WP_003890125.1
• Coordinates: 814864..815301 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A5N5UV41
• Locus tag: MPHLCCUG_RS04100
• Protein ID: WP_003890126.1
• Coordinates: 814655..814861 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPHLCCUG_RS04090	810674..812257	+	1584	WP_061482079.1	serine hydrolase	-
MPHLCCUG_RS04095	812254..814644	+	2391	WP_003890127.1	cation-translocating P-type ATPase	-
MPHLCCUG_RS04100	814655..814861	+	207	WP_003890126.1	antitoxin	Antitoxin
MPHLCCUG_RS04105	814864..815301	+	438	WP_003890125.1	SRPBCC family protein	Toxin
MPHLCCUG_RS04110	815329..816759	-	1431	WP_061482078.1	HNH endonuclease	-
MPHLCCUG_RS04115	816806..818269	-	1464	WP_061482101.1	long-chain fatty acid--CoA ligase	-
MPHLCCUG_RS04120	818266..819045	-	780	WP_061482077.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15751.34 Da
• Isoelectric Point: 10.1680

>T61244 WP_003890125.1 NZ_CP014475:814864-815301 [Mycolicibacterium phlei]
MAKLSVSVDVPLTPEQAWASASDLSRYKEWLSIHRVWRSKLPETLEKGTTLESIVEVMGMPNRIKWTIVHYRPPESMTLN
GNGVGGVKVKLIGKVKPAAEGSTVQFDIHLGGPALFGPIGMVVAGALRGDIQESLNRFKSVFAPA

Nucleotide

• Length: 438 bp

>T61244 NZ_CP014475:814864-815301 [Mycolicibacterium phlei]
ATGGCCAAACTCTCCGTCTCCGTCGACGTCCCGCTGACGCCGGAGCAGGCGTGGGCGTCCGCCTCTGACCTCTCGCGCTA
CAAGGAGTGGCTGTCCATCCACCGGGTGTGGCGCAGCAAGCTGCCCGAGACGCTGGAGAAGGGCACGACCCTGGAGTCCA
TCGTCGAGGTCATGGGCATGCCGAACCGGATCAAGTGGACCATCGTGCACTACCGTCCGCCGGAGTCGATGACGCTCAAC
GGCAACGGTGTCGGCGGTGTGAAGGTCAAGCTGATCGGCAAGGTGAAACCGGCAGCCGAGGGCTCGACCGTCCAGTTCGA
CATCCATCTCGGCGGGCCCGCACTGTTCGGACCGATCGGGATGGTGGTCGCCGGCGCACTGCGCGGCGATATCCAGGAGT
CGCTGAACCGCTTCAAGTCGGTTTTCGCTCCCGCCTAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7520.32 Da
• Isoelectric Point: 4.5859

>AT61244 WP_003890126.1 NZ_CP014475:814655-814861 [Mycolicibacterium phlei]
MSFLDKAKDLLAKNADKVETVIEKAGDIVDEKTEGKFAQHVDKVQEAAKNAAKNYVENLDNTENNPQS

Nucleotide

• Length: 207 bp

>AT61244 NZ_CP014475:814655-814861 [Mycolicibacterium phlei]
ATGTCATTCCTCGATAAGGCGAAGGACCTGCTGGCCAAGAACGCCGACAAGGTCGAGACGGTGATCGAGAAGGCCGGCGA
CATCGTCGACGAGAAGACCGAGGGCAAATTCGCCCAGCACGTCGACAAGGTCCAGGAAGCCGCCAAGAACGCCGCCAAGA
ACTACGTAGAGAACCTCGACAACACCGAGAACAACCCGCAGAGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5N5UV41