Detailed information of TA system TA061221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2235398..2235927
• Replicon: chromosome
• Accession: NZ_CP014444
• Organism: Staphylococcus aureus strain USA300-SUR24

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AYM37_RS11865
• Protein ID: WP_000621175.1
• Coordinates: 2235398..2235760 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AYM37_RS11870
• Protein ID: WP_000948331.1
• Coordinates: 2235757..2235927 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYM37_RS11840	2232376..2233146	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
AYM37_RS11845	2233121..2233600	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AYM37_RS11850	2233602..2233928	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AYM37_RS11855	2234047..2235048	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AYM37_RS11865	2235398..2235760	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYM37_RS11870	2235757..2235927	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AYM37_RS11875	2236012..2237160	-	1149	WP_001281145.1	alanine racemase	-
AYM37_RS11880	2237226..2237585	-	360	WP_000581200.1	holo-ACP synthase	-
AYM37_RS11885	2237589..2238080	-	492	WP_001205910.1	PH domain-containing protein	-
AYM37_RS11890	2238067..2239650	-	1584	WP_050961224.1	PH domain-containing protein	-
AYM37_RS11895	2239643..2240122	-	480	WP_001287088.1	hypothetical protein	-
AYM37_RS11900	2240330..2240890	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T61221 WP_000621175.1 NZ_CP014444:c2235760-2235398 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T61221 NZ_CP014444:c2235760-2235398 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT61221 WP_000948331.1 NZ_CP014444:c2235927-2235757 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT61221 NZ_CP014444:c2235927-2235757 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1